LOCUS       sequence-89051-e        2104 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2104)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..2104
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             30..683
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 cagatccgct agcgctaccg gtcgccacca tggtgagtgt gattaaacca gagatgaaga
       61 tgaggtacta catggacggc tccgtcaatg ggcatgagtt cacaattgaa ggtgaaggca
      121 caggcagacc ttacgaggga catcaagaga tgacactacg cgtcacaatg gccgagggcg
      181 ggccaatgcc tttcgcgttt gacttagtgt cacacgtgtt ctgttacggc cacagagtat
      241 ttactaaata tccagaagag ataccagact atttcaaaca agcatttcct gaaggcctgt
      301 catgggaaag gtcgttggag ttcgaagatg gtgggtccgc ttcagtcagt gcgcatataa
      361 gccttagagg aaacaccttc taccacaaat ccaaatttac tggggttaac tttcctgccg
      421 atggtcctat catgcaaaac caaagtgttg attgggagcc atcaaccgag aaaattactg
      481 ccagcgacgg agttctgaag ggtgatgtta cgatgtacct aaaacttgaa ggaggcggca
      541 atcacaaatg ccaaatgaag actacttaca aggcggcaaa agagattctt gaaatgccag
      601 gagaccatta catcggccat cgcctcgtca ggaaaaccga aggcaacatt actgagcagg
      661 tagaagatgc agtagctcat agctccggac tcagatctcg agtgcgtgag tgcatctcca
      721 tccacgttgg ccaggctggt gtccagattg gcaatgcctg ctgggagctc tactgcctgg
      781 aacacggcat ccagcccgat ggccagatgc caagtgacaa gaccattggg ggaggagatg
      841 actccttcaa caccttcttc agtgagacgg gcgctggcaa gcacgtgccc cgggctgtgt
      901 ttgtagactt ggaacccaca gtcattgatg aagttcgcac tggcacctac cgccagctct
      961 tccaccctga gcagctcatc acaggcaagg aagatgctgc caataactat gcccgagggc
     1021 actacaccat tggcaaggag atcattgacc ttgtgttgga ccgaattcgc aagctggctg
     1081 accagtgcac cggtcttcag ggcttcttgg ttttccacag ctttggtggg ggaactggtt
     1141 ctgggttcac ctccctgctc atggaacgtc tctcagttga ttatggcaag aagtccaagc
     1201 tggagttctc catttaccca gcaccccagg tttccacagc tgtagttgag ccctacaact
     1261 ccatcctcac cacccacacc accctggagc actctgattg tgccttcatg gtagacaatg
     1321 aggccatcta tgacatctgt cgtagaaacc tcgatatcga gcgcccaacc tacactaacc
     1381 ttaaccgcct tattagccag attgtgtcct ccatcactgc ttccctgaga tttgatggag
     1441 ccctgaatgt tgacctgaca gaattccaga ccaacctggt gccctacccc cgcatccact
     1501 tccctctggc cacatatgcc cctgtcatct ctgctgagaa agcctaccat gaacagcttt
     1561 ctgtagcaga gatcaccaat gcttgctttg agccagccaa ccagatggtg aaatgtgacc
     1621 ctcgccatgg taaatacatg gcttgctgcc tgttgtaccg tggtgacgtg gttcccaaag
     1681 atgtcaatgc tgccattgcc accatcaaaa ccaagcgcag catccagttt gtggattggt
     1741 gccccactgg cttcaaggtt ggcatcaact accagcctcc cactgtggtg cctggtggag
     1801 acctggccaa ggtacagaga gctgtgtgca tgctgagcaa caccacagcc attgctgagg
     1861 cctgggctcg cctggaccac aagtttgacc tgatgtatgc caagcgtgcc tttgttcact
     1921 ggtacgtggg tgaggggatg gaggaaggcg agttttcaga ggcccgtgaa gatatggctg
     1981 cccttgagaa ggattatgag gaggttggtg tggattctgt tgaaggagag ggtgaggaag
     2041 aaggagagga atactaagga tccaccggat ctagataact gatcataatc agccatacca
     2101 catt
//
