LOCUS       sequence-89052-e        2144 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2144)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Tubulin-C-18
FEATURES             Location/Qualifiers
     source          1..2144
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..687
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gtgtgattaa accagagatg
       61 aagatgaggt actacatgga cggctccgtc aatgggcatg agttcacaat tgaaggtgaa
      121 ggcacaggca gaccttacga gggacatcaa gagatgacac tacgcgtcac aatggccgag
      181 ggcgggccaa tgcctttcgc gtttgactta gtgtcacacg tgttctgtta cggccacaga
      241 gtatttacta aatatccaga agagatacca gactatttca aacaagcatt tcctgaaggc
      301 ctgtcatggg aaaggtcgtt ggagttcgaa gatggtgggt ccgcttcagt cagtgcgcat
      361 ataagcctta gaggaaacac cttctaccac aaatccaaat ttactggggt taactttcct
      421 gccgatggtc ctatcatgca aaaccaaagt gttgattggg agccatcaac cgagaaaatt
      481 actgccagcg acggagttct gaagggtgat gttacgatgt acctaaaact tgaaggaggc
      541 ggcaatcaca aatgccaaat gaagactact tacaaggcgg caaaagagat tcttgaaatg
      601 ccaggagacc attacatcgg ccatcgcctc gtcaggaaaa ccgaaggcaa cattactgag
      661 caggtagaag atgcagtagc tcatagctcc ggactcagat ctggcagcgg tggaggcagc
      721 gcatccggcg gaagcggaag cgtgcgtgag tgcatctcca tccacgttgg ccaggctggt
      781 gtccagattg gcaatgcctg ctgggagctc tactgcctgg aacacggcat ccagcccgat
      841 ggccagatgc caagtgacaa gaccattggg ggaggagatg actccttcaa caccttcttc
      901 agtgagacgg gcgctggcaa gcacgtgccc cgggctgtgt ttgtagactt ggaacccaca
      961 gtcattgatg aagttcgcac tggcacctac cgccagctct tccaccctga gcagctcatc
     1021 acaggcaagg aagatgctgc caataactat gcccgagggc actacaccat tggcaaggag
     1081 atcattgacc ttgtgttgga ccgaattcgc aagctggctg accagtgcac cggtcttcag
     1141 ggcttcttgg ttttccacag ctttggtggg ggaactggtt ctgggttcac ctccctgctc
     1201 atggaacgtc tctcagttga ttatggcaag aagtccaagc tggagttctc catttaccca
     1261 gcaccccagg tttccacagc tgtagttgag ccctacaact ccatcctcac cacccacacc
     1321 accctggagc actctgattg tgccttcatg gtagacaatg aggccatcta tgacatctgt
     1381 cgtagaaacc tcgatatcga gcgcccaacc tacactaacc ttaaccgcct tattagccag
     1441 attgtgtcct ccatcactgc ttccctgaga tttgatggag ccctgaatgt tgacctgaca
     1501 gaattccaga ccaacctggt gccctacccc cgcatccact tccctctggc cacatatgcc
     1561 cctgtcatct ctgctgagaa agcctaccat gaacagcttt ctgtagcaga gatcaccaat
     1621 gcttgctttg agccagccaa ccagatggtg aaatgtgacc ctcgccatgg taaatacatg
     1681 gcttgctgcc tgttgtaccg tggtgacgtg gttcccaaag atgtcaatgc tgccattgcc
     1741 accatcaaaa ccaagcgcag catccagttt gtggattggt gccccactgg cttcaaggtt
     1801 ggcatcaact accagcctcc cactgtggtg cctggtggag acctggccaa ggtacagaga
     1861 gctgtgtgca tgctgagcaa caccacagcc attgctgagg cctgggctcg cctggaccac
     1921 aagtttgacc tgatgtatgc caagcgtgcc tttgttcact ggtacgtggg tgaggggatg
     1981 gaggaaggcg agttttcaga ggcccgtgaa gatatggctg cccttgagaa ggattatgag
     2041 gaggttggtg tggattctgt tgaaggagag ggtgaggaag aaggagagga atactaagga
     2101 tccaccggat ctagataact gatcataatc agccatacca catt
//
