LOCUS       sequence-89053-e        1873 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1873)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-VASP-5
FEATURES             Location/Qualifiers
     source          1..1873
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..688
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agtgtgatta aaccagagat
       61 gaagatgagg tactacatgg acggctccgt caatgggcat gagttcacaa ttgaaggtga
      121 aggcacaggc agaccttacg agggacatca agagatgaca ctacgcgtca caatggccga
      181 gggcgggcca atgcctttcg cgtttgactt agtgtcacac gtgttctgtt acggccacag
      241 agtatttact aaatatccag aagagatacc agactatttc aaacaagcat ttcctgaagg
      301 cctgtcatgg gaaaggtcgt tggagttcga agatggtggg tccgcttcag tcagtgcgca
      361 tataagcctt agaggaaaca ccttctacca caaatccaaa tttactgggg ttaactttcc
      421 tgccgatggt cctatcatgc aaaaccaaag tgttgattgg gagccatcaa ccgagaaaat
      481 tactgccagc gacggagttc tgaagggtga tgttacgatg tacctaaaac ttgaaggagg
      541 cggcaatcac aaatgccaaa tgaagactac ttacaaggcg gcaaaagaga ttcttgaaat
      601 gccaggagac cattacatcg gccatcgcct cgtcaggaaa accgaaggca acattactga
      661 gcaggtagaa gatgcagtag ctcatagctc cggactcaga tctatgagcg agacggtcat
      721 ctgttccagc cgggctactg tgatgcttta tgatgacagc aacaagcgat ggctccctgc
      781 tggcactggt ccccaggcct tcagccgcgt ccagatatac cacaacccca ctgctaactc
      841 cttccgagtt gttggccgca agatgcagcc ggatcagcag gtggttatca actgtgccat
      901 cattcggggt gtcaagtaca atcaggccac tcccatcttc catcagtggc gagatgcccg
      961 ccaggtctgg ggcctcaact tcggcagcaa ggaggacgcc atacagtttg caacaggcat
     1021 ggccaacgcc ctagaggcct tggaaggagg tgggcctccc ccagccccag caccccctgc
     1081 ctggtctgcc cagaatggtc cctccccaga ggagctggaa caacagaaaa ggcagccgga
     1141 gcatatggag cgccgggtct ccaatgcagg aggcccacct gctcccccag ctgggggccc
     1201 tcctccacct ccaggacctc cccctcctcc aggtcccccc ccacccccag gtctgccctc
     1261 ctcaggggta tctggggcag gtcatggagc aggggcagcc ccaccccctg cacccccact
     1321 ccctacaaca cagggcccca atagtggggg ttccggggcc ccaggcctgg ctgctgccat
     1381 tgctggagcc aaactcagga aagtgagcaa gcaggaggag gcctctgggg ggcccctggc
     1441 ccccaaagct gagaacagtc gaagcactgg tggggggctt atggaagaga tgaacgccat
     1501 gctggcccgg agaagaaaag ccacacaggt tggggagaag ccccccaaag acgagtcagc
     1561 cagtgaggag tcagaggccc gactccctgc ccagagtgaa cctgtgagaa gaccctggga
     1621 gaagaacagc acaaccttgc caaggatgaa gtcgtcctct tctgtgacta cctccgaggc
     1681 ccacccctcg acgccctgct ccagtgatga ctccgacttg gagagggtga agcaggagct
     1741 tctggaagag gtgcggaagg agctacagaa aatgaaagag gaaatcatcg aagtctttgt
     1801 ccaggagctg aggaagcggg gttctccttg agcggccgcg actctagatc ataatcagcc
     1861 ataccacatt gag
//
