LOCUS       sequence-89057-e        2466 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 551, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2466)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKO2-Zyxin-6
FEATURES             Location/Qualifiers
     source          1..2466
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1761..1770
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1767..2423
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAHS"
ORIGIN
        1 gactcagatc tcgagctcaa gcttcgaatt ccatggcggc cccccgcccg tctcccgcga
       61 tccccgtttc ggtctcggct ccggcttttt acgccccgca gaagaagttc ggccctgtgg
      121 tggccccaaa gcccaaagtg aatcccttcc ggcccaggga cagcgagcct cccccggcac
      181 ccggggccca gcgcgcacag atgggccggg tgggcgatat tcccccgccg cccccggaag
      241 actttcccct gcctccacct ccccttgctg gggatggcga cgatgcagag ggtgctctgg
      301 gaggtgcctt cccgccgccc cctcccccga tcgaggaatc atttccccct gcgcctctgg
      361 aggaggagat cttcccttcc ccgccgcctc ctccggagga ggagggaggg cctgaggccc
      421 ccataccgcc cccaccacag cccagggaga aggtgagcag tattgatttg gagatcgact
      481 ctctgtcctc actgctggat gacatgacca agaatgatcc tttcaaagcc cgggtgtcat
      541 ctggatatgt gcccccacca gtggccactc cattcagttc caagtccagt accaagcctg
      601 cagccggggg cacagcaccc ctgcctcctt ggaagtcccc ttccagctcc cagcctctgc
      661 cccaggttcc ggctccggct cagagccaga cacagttcca tgttcagccc cagccccagc
      721 ccaagcctca ggtccaactc catgtccagt cccagaccca gcctgtgtct ttggctaaca
      781 cccagccccg agggccccca gcctcatctc cggctccagc ccctaagttt tctccagtga
      841 ctcctaagtt tactcctgtg gcttccaagt tcagtcctgg agccccaggt ggatctgggt
      901 cacaaccaaa tcaaaaattg gggcaccccg aagctctttc tgctggcaca ggctcccctc
      961 aacctcccag cttcacctat gcccagcaga gggagaagcc ccgagtgcag gagaagcagc
     1021 accccgtgcc cccaccggct cagaaccaaa accaggtgcg ctcccctggg gccccagggc
     1081 ccctgactct gaaggaggtg gaggagctgg agcagctgac ccagcagcta atgcaggaca
     1141 tggagcatcc tcagaggcag aatgtggctg tcaacgaact ctgcggccga tgccatcaac
     1201 ccctggcccg ggcgcagcca gccgtccgcg ctctagggca gctgttccac atcgcctgct
     1261 tcacctgcca ccagtgtgcg cagcagctcc agggccagca gttctacagt ctggaggggg
     1321 cgccgtactg cgagggctgt tacactgaca ccctggagaa gtgtaacacc tgcggggagc
     1381 ccatcactga ccgcatgctg agggccacgg gcaaggccta tcacccgcac tgcttcacct
     1441 gtgtggtctg cgcccgcccc ctggagggca cctccttcat cgtggaccag gccaaccggc
     1501 cccactgtgt ccccgactac cacaagcagt acgccccgag gtgctccgtc tgctctgagc
     1561 ccatcatgcc tgagcctggc cgagatgaga ctgtgcgagt ggtcgccctg gacaagaact
     1621 tccacatgaa gtgttacaag tgtgaggact gcgggaagcc cctgtcgatt gaggcagatg
     1681 acaatggctg cttccccctg gacggtcacg tgctctgtcg gaagtgccac actgctagag
     1741 cccagacgga tccaccggtc gccaccatgg tgagtgtgat taaaccagag atgaagatga
     1801 ggtactacat ggacggctcc gtcaatgggc atgagttcac aattgaaggt gaaggcacag
     1861 gcagacctta cgagggacat caagagatga cactacgcgt cacaatggcc gagggcgggc
     1921 caatgccttt cgcgtttgac ttagtgtcac acgtgttctg ttacggccac agagtattta
     1981 ctaaatatcc agaagagata ccagactatt tcaaacaagc atttcctgaa ggcctgtcat
     2041 gggaaaggtc gttggagttc gaagatggtg ggtccgcttc agtcagtgcg catataagcc
     2101 ttagaggaaa caccttctac cacaaatcca aatttactgg ggttaacttt cctgccgatg
     2161 gtcctatcat gcaaaaccaa agtgttgatt gggagccatc aaccgagaaa attactgcca
     2221 gcgacggagt tctgaagggt gatgttacga tgtacctaaa acttgaagga ggcggcaatc
     2281 acaaatgcca aatgaagact acttacaagg cggcaaaaga gattcttgaa atgccaggag
     2341 accattacat cggccatcgc ctcgtcagga aaaccgaagg caacattact gagcaggtag
     2401 aagatgcagt agctcatagc taagcggccg cgactctaga tcataatcag ccataccaca
     2461 ttgaga
//
