LOCUS       sequence-89058-e        1933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1933)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-Actin-7
FEATURES             Location/Qualifiers
     source          1..1933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             62..712
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaataa
       61 catggccgtg attaaaccag agatgaagat gaggtactac atggacggct ccgtcaatgg
      121 gcatgagttc acaattgaag gtgaaggcac aggcagacct tacgagggac atcaagagat
      181 gacactacgc gtcacaatgg ccaagggcgg gccaatgcct ttcgcgtttg acttagtgtc
      241 acacgtgttc tgttacggcc acagaccttt tactaaatat ccagaagaga taccagacta
      301 tttcaaacaa gcatttcctg aaggcctgtc atgggaaagg tcgttggagt tcgaagatgg
      361 tgggtccgct tcagtcagtg cgcatataag ccttagagga aacaccttct accacaaatc
      421 caaatttact ggggttaact ttcctgccga tggtcctatc atgcaaaacc aaagtgttga
      481 ttgggagcca tcaaccgaga aaattactgc cagcgacgga gttctgaagg gtgatgttac
      541 gatgtaccta aaacttgaag gaggcggcaa tcacaaatgc caattcaaga ctacttacaa
      601 ggcggcaaaa aagattctta aaatgccagg aagccattac atcagccatc gcctcgtcag
      661 gaaaaccgaa ggcaacatta ctgagctggt agaagatgca gtagctcatt ccggcggcat
      721 ggacgagctg tacaagtccg gactcagatc tcgagctatg gatgatgata tcgccgcgct
      781 cgtcgtcgac aacggctccg gcatgtgcaa ggccggcttc gcgggcgacg atgccccccg
      841 ggccgtcttc ccctccatcg tggggcgccc caggcaccag ggcgtgatgg tgggcatggg
      901 tcagaaggat tcctatgtgg gcgacgaggc ccagagcaag agaggcatcc tcaccctgaa
      961 gtaccccatc gagcacggca tcgtcaccaa ctgggacgac atggagaaaa tctggcacca
     1021 caccttctac aatgagctgc gtgtggctcc cgaggagcac cccgtgctgc tgaccgaggc
     1081 ccccctgaac cccaaggcca accgcgagaa gatgacccag atcatgtttg agaccttcaa
     1141 caccccagcc atgtacgttg ctatccaggc tgtgctatcc ctgtacgcct ctggccgtac
     1201 cactggcatc gtgatggact ccggtgacgg ggtcacccac actgtgccca tctacgaggg
     1261 gtatgccctc ccccatgcca tcctgcgtct ggacctggct ggccgggacc tgactgacta
     1321 cctcatgaag atcctcaccg agcgcggcta cagcttcacc accacggccg agcgggaaat
     1381 cgtgcgtgac attaaggaga agctgtgcta cgtcgccctg gacttcgagc aagagatggc
     1441 cacggctgct tccagctcct ccctggagaa gagctacgag ctgcctgacg gccaggtcat
     1501 caccattggc aatgagcggt tccgctgccc tgaggcactc ttccagcctt ccttcctggg
     1561 catggagtcc tgtggcatcc acgaaactac cttcaactcc atcatgaagt gtgacgtgga
     1621 catccgcaaa gacctgtacg ccaacacagt gctgtctggc ggcaccacca tgtaccctgg
     1681 cattgccgac aggatgcaga aggagatcac tgccctggca cccagcacaa tgaagatcaa
     1741 gatcattgct cctcctgagc gcaagtactc cgtgtggatc ggcggctcca tcctggcctc
     1801 gctgtccacc ttccagcaga tgtggatcag caagcaggag tatgacgagt ccggcccctc
     1861 catcgtccac cgcaaatgct tctagggatc caccggatct agataactga tcataatcag
     1921 ccataccaca ttg
//
