LOCUS       sequence-89059-e        3520 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Alpha Actinin, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3520)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 3520)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-Alpha-Actinin-19
FEATURES             Location/Qualifiers
     source          1..3520
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      2771..2780
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2804..3454
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gcaccatcat
       61 ggaccattat gattctcagc aaaccaacga ttacatgcag ccagaagagg actgggaccg
      121 ggacctgctc ctggacccgg cctgggagaa gcagcagaga aagacattca cggcatggtg
      181 taactcccac ctccggaagg cggggacaca gatcgagaac atcgaagagg acttccggga
      241 tggcctgaag ctcatgctgc tgctggaggt catctcaggt gaacgcttgg ccaagccaga
      301 gcgaggcaag atgagagtgc acaagatctc caacgtcaac aaggccctgg atttcatagc
      361 cagcaaaggc gtcaaactgg tgtccatcgg agccgaagaa atcgtggatg ggaatgtgaa
      421 gatgaccctg ggcatgatct ggaccatcat cctgcgcttt gccatccagg acatctccgt
      481 ggaagagact tcagccaagg aagggctgct cctgtggtgt cagagaaaga cagcccctta
      541 caaaaatgtc aacatccaga acttccacat aagctggaag gatggcctcg gcttctgtgc
      601 tttgatccac cgacaccggc ccgagctgat tgactacggg aagctgcgga aggatgatcc
      661 actcacaaat ctgaatacgg cttttgacgt ggcagagaag tacctggaca tccccaagat
      721 gctggatgcc gaagacatcg ttggaactgc ccgaccggat gagaaagcca tcatgactta
      781 cgtgtctagc ttctaccacg ccttctctgg agcccagaag gcggagacag cagccaatcg
      841 catctgcaag gtgttggccg tcaaccagga gaacgagcag cttatggaag actacgagaa
      901 gctggccagt gatctgttgg agtggatccg ccgcacaatc ccgtggctgg agaaccgggt
      961 gcccgagaac accatgcatg ccatgcaaca gaagctggag gacttccggg actaccggcg
     1021 cctgcacaag ccgcccaagg tgcaggagaa gtgccagctg gagatcaact tcaacacgct
     1081 gcagaccaag ctgcggctca gcaaccggcc tgccttcatg ccctctgagg gcaggatggt
     1141 ctcggacatc aacaatgcct ggggctgcct ggagcaggtg gagaagggct atgaggagtg
     1201 gttgctgaat gagatccgga ggctggagcg actggaccac ctggcagaga agttccggca
     1261 gaaggcctcc atccacgagg cctggactga cggcaaagag gccatgctgc gacagaagga
     1321 ctatgagacc gccaccctct cggagatcaa ggccttgctc aagaagcatg aggccttcga
     1381 gagtgacctg gctgcccacc aggaccgtgt ggagcagatt gccgccatcg cacaggagct
     1441 caatgagctg gactattatg actcacccag tgtcaacgcc cgttgccaaa agatctgtga
     1501 ccagtgggac aatctggggg ccctaactca gaagcgaagg gaagctctgg agcggaccga
     1561 gaaactgctg gagaccattg accagctgta cttggagtat gccaagcggg ctgcaccctt
     1621 caacaactgg atggaggggg ccatggagga cctgcaggac accttcattg tgcacaccat
     1681 tgaggagatc cagggactga ccacagccca tgagcagttc aaggccaccc tccctgatgc
     1741 cgacaaggag cgcctggcca tcctgggcat ccacaatgag gtgtccaaga ttgtccagac
     1801 ctaccacgtc aatatggcgg gcaccaaccc ctacacaacc atcacgcctc aggagatcaa
     1861 tggcaaatgg gaccacgtgc ggcagctggt gcctcggagg gaccaagctc tgacggagga
     1921 gcatgcccga cagcagcaca atgagaggct acgcaagcag tttggagccc aggccaatgt
     1981 catcgggccc tggatccaga ccaagatgga ggagatcggg aggatctcca ttgagatgca
     2041 tgggaccctg gaggaccagc tcagccacct gcggcagtat gagaagagca tcgtcaacta
     2101 caagccaaag attgatcagc tggagggcga ccaccagctc atccaggagg cgctcatctt
     2161 cgacaacaag cacaccaact acaccatgga gcacatccgt gtgggctggg agcagctgct
     2221 caccaccatc gccaggacca tcaatgaggt agagaaccag atcctgaccc gggatgccaa
     2281 gggcatcagc caggagcaga tgaatgagtt ccgggcctcc ttcaaccact ttgaccggaa
     2341 gaagacaggc atgatggaca cggatgattt ccgcgcctgc ctgatctcca tgggttacaa
     2401 catgggagaa gcagaatttg cccgcatcat gagcattgtg gaccccaacc gcctgggggt
     2461 agtgacattc caggccttca ttgacttcat gtcccgcgag acagccgaca cagatacagc
     2521 agaccaagtc atggcttcct tcaagatcct ggctggggac aagaactaca ttaccatgga
     2581 cgagctgcgc cgcgagctgc cacccgacca ggctgagtac tgcatcgcgc ggatggcccc
     2641 ctacaccggc cccgactccg tgccaggtgc tctggactac atgtccttct ccacggcgct
     2701 gtacggcgag agtgacctca agcttcgaat tctgcagtcg acggtaccgc gggcccggga
     2761 tccaccggtc gccaccatgg tgagcaaggg cgaggagaat aacatggccg tgattaaacc
     2821 agagatgaag atgaggtact acatggacgg ctccgtcaat gggcatgagt tcacaattga
     2881 aggtgaaggc acaggcagac cttacgaggg acatcaagag atgacactac gcgtcacaat
     2941 ggccaagggc gggccaatgc ctttcgcgtt tgacttagtg tcacacgtgt tctgttacgg
     3001 ccacagacct tttactaaat atccagaaga gataccagac tatttcaaac aagcatttcc
     3061 tgaaggcctg tcatgggaaa ggtcgttgga gttcgaagat ggtgggtccg cttcagtcag
     3121 tgcgcatata agccttagag gaaacacctt ctaccacaaa tccaaattta ctggggttaa
     3181 ctttcctgcc gatggtccta tcatgcaaaa ccaaagtgtt gattgggagc catcaaccga
     3241 gaaaattact gccagcgacg gagttctgaa gggtgatgtt acgatgtacc taaaacttga
     3301 aggaggcggc aatcacaaat gccaattcaa gactacttac aaggcggcaa aaaagattct
     3361 taaaatgcca ggaagccatt acatcagcca tcgcctcgtc aggaaaaccg aaggcaacat
     3421 tactgagctg gtagaagatg cagtagctca ttccggcggc atggacgagc tgtacaagta
     3481 agcggccgcg actctagatc ataatcagcc ataccacatt
//
