LOCUS       sequence-89060-e        1249 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centromere Protein A, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1249)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-CENPA-N-22
FEATURES             Location/Qualifiers
     source          1..1249
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    440..492
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      500..509
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             533..1183
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 gtcagatccg ctagccacca tgggcccgcg ccgccggagc cgaaagcccg aggccccgag
       61 gaggcgcagc ccgagcccga ccccgacccc cggcccctcc cggcggggcc cctccttagg
      121 cgcttcctcc catcaacaca gtcggcggag acaaggttgg ctaaaggaga tccgaaagct
      181 tcagaagagc acacacctct tgataaggaa gctgcccttc agccgcctgg caagagaaat
      241 atgtgttaaa ttcactcgtg gtgtggactt caattggcaa gcccaggccc tattggccct
      301 acaagaggca gcagaagcat ttctagttca tctctttgag gacgcctatc tcctcacctt
      361 acatgcaggc cgagttactc tcttcccaaa ggatgtgcaa ctggcccgga ggatccgggg
      421 ccttgaggag ggactcggcc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg
      481 ggcccgggat ccaccggtcg ccaccatggt gagcaagggc gaggagaata acatggccgt
      541 gattaaacca gagatgaaga tgaggtacta catggacggc tccgtcaatg ggcatgagtt
      601 cacaattgaa ggtgaaggca caggcagacc ttacgaggga catcaagaga tgacactacg
      661 cgtcacaatg gccaagggcg ggccaatgcc tttcgcgttt gacttagtgt cacacgtgtt
      721 ctgttacggc cacagacctt ttactaaata tccagaagag ataccagact atttcaaaca
      781 agcatttcct gaaggcctgt catgggaaag gtcgttggag ttcgaagatg gtgggtccgc
      841 ttcagtcagt gcgcatataa gccttagagg aaacaccttc taccacaaat ccaaatttac
      901 tggggttaac tttcctgccg atggtcctat catgcaaaac caaagtgttg attgggagcc
      961 atcaaccgag aaaattactg ccagcgacgg agttctgaag ggtgatgtta cgatgtacct
     1021 aaaacttgaa ggaggcggca atcacaaatg ccaattcaag actacttaca aggcggcaaa
     1081 aaagattctt aaaatgccag gaagccatta catcagccat cgcctcgtca ggaaaaccga
     1141 aggcaacatt actgagctgg tagaagatgc agtagctcat tccggcggca tggacgagct
     1201 gtacaagtaa gcggccgcga ctctagatca taatcagcca taccacatt
//
