LOCUS       sequence-89061-e        1323 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1323)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..1323
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    523..575
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      583..592
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             616..1266
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 agatccgcta gcaccatggg ccccaagcgg cggcagctga cgttccggga gaagtcgcgg
       61 atcatccagg aggtggagga gaacccggac ctacgcaagg gcgagatcgc gcggcgcttc
      121 aacatcccgc cgtccacgct gagcacgatc ctgaagaaca agcgcgccat cctggcgtcg
      181 gagcgcaagt acggggtggc ctccacctgc cgcaagacca acaagctgtc tccctacgac
      241 aagctcgagg gcttgctcat cgcctggttc cagcagatcc gcgccgccgg cctgccggtc
      301 aagggcatca tcctcaagga gaaggcgctg cgcatagccg aggagctggg catggacgac
      361 ttcaccgcct ccaacggctg gctggaccgc ttccgccggc gccacggcgt ggtgtcctgc
      421 agcggcgtgg cccgcgcccg cgcgcgaaac gctgcccccc gcaccccggc ggcgcctgcc
      481 agtccggccg cggtgccctc ggagggcagt ggcgggagca ctctcgagct caagcttcga
      541 attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag
      601 ggcgaggaga ataacatggc cgtgattaaa ccagagatga agatgaggta ctacatggac
      661 ggctccgtca atgggcatga gttcacaatt gaaggtgaag gcacaggcag accttacgag
      721 ggacatcaag agatgacact acgcgtcaca atggccaagg gcgggccaat gcctttcgcg
      781 tttgacttag tgtcacacgt gttctgttac ggccacagac cttttactaa atatccagaa
      841 gagataccag actatttcaa acaagcattt cctgaaggcc tgtcatggga aaggtcgttg
      901 gagttcgaag atggtgggtc cgcttcagtc agtgcgcata taagccttag aggaaacacc
      961 ttctaccaca aatccaaatt tactggggtt aactttcctg ccgatggtcc tatcatgcaa
     1021 aaccaaagtg ttgattggga gccatcaacc gagaaaatta ctgccagcga cggagttctg
     1081 aagggtgatg ttacgatgta cctaaaactt gaaggaggcg gcaatcacaa atgccaattc
     1141 aagactactt acaaggcggc aaaaaagatt cttaaaatgc caggaagcca ttacatcagc
     1201 catcgcctcg tcaggaaaac cgaaggcaac attactgagc tggtagaaga tgcagtagct
     1261 cattccggcg gcatggacga gctgtacaag taagcggccg cgactctaga tcataatcag
     1321 cca
//
