LOCUS       sequence-89063-e        1541 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1541)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-Cx26-7
FEATURES             Location/Qualifiers
     source          1..1541
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      791..800
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             824..1474
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagctcaagc ttcgaattcg cggccgcacg
       61 ctcctcggga cacagtgcca accatccaga ggacaagatg gattggggca cactacagag
      121 catcctcggg ggtgtcaaca agcactccac cagcattggg aaaatctggc tcactgtcct
      181 cttcatcttc cgcatcatga tcctcgtggt ggccgcgaag gaggtgtggg gagatgagca
      241 agccgatttt gtttgcaaca ctctccagcc tggctgtaag aatgtgtgct acgaccacta
      301 cttccccatc tctcacatcc ggctctgggc tctgcagctg atcatggtgt ccacgccggc
      361 cctcctggta gctatgcacg tggcctaccg gagacacgaa aagaaacgga agttcatgaa
      421 gggagagata aagaacgagt ttaaggacat cgaagagatc aaaacccaga aggtccgtat
      481 cgaagggtcc ctgtggtgga cctacaccac cagcatcttc ttccgggtca tcttcgaagc
      541 tgtcttcatg tatgtctttt acatcatgta caatggcttc ttcatgcagc gtctggtgaa
      601 gtgtaacgcc tggccttgtc ccaatacagt ggactgcttc atttccaggc ccacagaaaa
      661 gactgtcttc acggtgttca tgatctctgt gtctggaatt tgcatcctgc taaacatcac
      721 agagctgtgc tatctgttca ttaggtattg ctcagggaag tccaaaagac cagtcgcgga
      781 tccaccggtc gccaccatgg tgagcaaggg cgaggagaat aacatggccg tgattaaacc
      841 agagatgaag atgaggtact acatggacgg ctccgtcaat gggcatgagt tcacaattga
      901 aggtgaaggc acaggcagac cttacgaggg acatcaagag atgacactac gcgtcacaat
      961 ggccaagggc gggccaatgc ctttcgcgtt tgacttagtg tcacacgtgt tctgttacgg
     1021 ccacagacct tttactaaat atccagaaga gataccagac tatttcaaac aagcatttcc
     1081 tgaaggcctg tcatgggaaa ggtcgttgga gttcgaagat ggtgggtccg cttcagtcag
     1141 tgcgcatata agccttagag gaaacacctt ctaccacaaa tccaaattta ctggggttaa
     1201 ctttcctgcc gatggtccta tcatgcaaaa ccaaagtgtt gattgggagc catcaaccga
     1261 gaaaattact gccagcgacg gagttctgaa gggtgatgtt acgatgtacc taaaacttga
     1321 aggaggcggc aatcacaaat gccaattcaa gactacttac aaggcggcaa aaaagattct
     1381 taaaatgcca ggaagccatt acatcagcca tcgcctcgtc aggaaaaccg aaggcaacat
     1441 tactgagctg gtagaagatg cagtagctca ttccggcggc atggacgagc tgtacaagta
     1501 agcggccgcg actctagatc ataatcagcc ataccacatt g
//
