LOCUS       sequence-89064-e        1722 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1722)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-Cx32-7
FEATURES             Location/Qualifiers
     source          1..1722
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      972..981
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1005..1655
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 cgctagcgct accggactca gatctcgagc tcaagcttcg aattccctct gggaaagggc
       61 agcagcagcc aggtgtggca gtgacaggga ggtgtgaatg aggcaggatg aactggacag
      121 gtttgtacac cttgctcagt ggcgtgaacc ggcattctac tgccattggc cgagtatggc
      181 tctcggtcat cttcatcttc agaatcatgg tgctggtggt ggctgcagag agtgtgtggg
      241 gtgatgagaa atcttccttc atctgcaaca cactccagcc tggctgcaac agcgtttgct
      301 atgaccaatt cttccccatc tcccatgtgc ggctgtggtc cctgcagctc atcctagttt
      361 ccaccccagc tctcctcgtg gccatgcacg tggctcacca gcaacacata gagaagaaaa
      421 tgctacggct tgagggccat ggggaccccc tacacctgga ggaggtgaag aggcacaagg
      481 tccacatctc agggacactg tggtggacct atgtcatcag cgtggtgttc cggctgttgt
      541 ttgaggccgt cttcatgtat gtcttttatc tgctctaccc tggctatgcc atggtgcggc
      601 tggtcaagtg cgacgtctac ccctgcccca acacagtgga ctgcttcgtg tcccgcccca
      661 ccgagaaaac cgtcttcacc gtcttcatgc tagctgcctc tggcatctgc atcatcctca
      721 atgtggccga ggtggtgtac ctcatcatcc gggcctgtgc ccgccgagcc cagcgccgct
      781 ccaatccacc ttcccgcaag ggctcgggct tcggccaccg cctctcacct gaatacaagc
      841 agaatgagat caacaagctg ctgagtgagc aggatggctc cctgaaagac atactgcgcc
      901 gcagccctgg caccggggct gggctggctg aaaagagcga ccgctgctcg gcctgcgcgg
      961 atccaccggt cgccaccatg gtgagcaagg gcgaggagaa taacatggcc gtgattaaac
     1021 cagagatgaa gatgaggtac tacatggacg gctccgtcaa tgggcatgag ttcacaattg
     1081 aaggtgaagg cacaggcaga ccttacgagg gacatcaaga gatgacacta cgcgtcacaa
     1141 tggccaaggg cgggccaatg cctttcgcgt ttgacttagt gtcacacgtg ttctgttacg
     1201 gccacagacc ttttactaaa tatccagaag agataccaga ctatttcaaa caagcatttc
     1261 ctgaaggcct gtcatgggaa aggtcgttgg agttcgaaga tggtgggtcc gcttcagtca
     1321 gtgcgcatat aagccttaga ggaaacacct tctaccacaa atccaaattt actggggtta
     1381 actttcctgc cgatggtcct atcatgcaaa accaaagtgt tgattgggag ccatcaaccg
     1441 agaaaattac tgccagcgac ggagttctga agggtgatgt tacgatgtac ctaaaacttg
     1501 aaggaggcgg caatcacaaa tgccaattca agactactta caaggcggca aaaaagattc
     1561 ttaaaatgcc aggaagccat tacatcagcc atcgcctcgt caggaaaacc gaaggcaaca
     1621 ttactgagct ggtagaagat gcagtagctc attccggcgg catggacgag ctgtacaagt
     1681 aagcggccgc gactctagat cataatcagc cataccacat tt
//
