LOCUS       sequence-89065-e        1665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 548, Emission: 
            561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1665)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-EB3-7
FEATURES             Location/Qualifiers
     source          1..1665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      52..61
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      916..925
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             949..1599
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 gcgctaccgg actcagatct cgagctcaag cttcgaattc tgcagtcgac tgccaccatg
       61 gccgtcaatg tgtactccac atctgtgacc agtgaaaatc tgagtcgcca tgatatgctt
      121 gcatgggtca acgactccct gcacctcaac tataccaaga tagaacagct ttgttcaggg
      181 gcagcctact gccagttcat ggacatgctc ttccccggct gtgtgcactt gaggaaagtg
      241 aagttccagg ccaaactaga gcatgaatac atccacaact tcaaggtgct gcaagcagct
      301 ttcaagaaga tgggtgttga caaaatcatt cctgtagaga aattagtgaa aggaaaattc
      361 caagataatt ttgagtttat tcagtggttt aagaaattct ttgacgcaaa ctatgatgga
      421 aaggattaca accctctgct ggcgcggcag ggccaggacg tagcgccacc tcctaaccca
      481 ggtgatcaga tcttcaacaa atccaagaaa ctcattggca cagcagttcc acagaggacg
      541 tcccccacag gcccaaaaaa catgcagacc tctggccggc tgagcaatgt ggcccccccc
      601 tgcattctcc ggaagaatcc tccatcagcc cgaaatggcg gccatgagac tgatgcccaa
      661 attcttgaac tcaaccaaca gctggtggac ttgaagctga cagtggatgg gctggagaag
      721 gaacgtgact tctacttcag caaacttcgt gacatcgagc tcatctgcca ggagcatgaa
      781 agtgaaaaca gccctgttat ctcaggcatc attggcatcc tctatgccac agaggaagga
      841 ttcgcacccc ctgaggacga tgagattgaa gagcatcaac aagaagacca ggacgagtac
      901 ctggatccac cggtcgccac catggtgagc aagggcgagg agaataacat ggccgtgatt
      961 aaaccagaga tgaagatgag gtactacatg gacggctccg tcaatgggca tgagttcaca
     1021 attgaaggtg aaggcacagg cagaccttac gagggacatc aagagatgac actacgcgtc
     1081 acaatggcca agggcgggcc aatgcctttc gcgtttgact tagtgtcaca cgtgttctgt
     1141 tacggccaca gaccttttac taaatatcca gaagagatac cagactattt caaacaagca
     1201 tttcctgaag gcctgtcatg ggaaaggtcg ttggagttcg aagatggtgg gtccgcttca
     1261 gtcagtgcgc atataagcct tagaggaaac accttctacc acaaatccaa atttactggg
     1321 gttaactttc ctgccgatgg tcctatcatg caaaaccaaa gtgttgattg ggagccatca
     1381 accgagaaaa ttactgccag cgacggagtt ctgaagggtg atgttacgat gtacctaaaa
     1441 cttgaaggag gcggcaatca caaatgccaa ttcaagacta cttacaaggc ggcaaaaaag
     1501 attcttaaaa tgccaggaag ccattacatc agccatcgcc tcgtcaggaa aaccgaaggc
     1561 aacattactg agctggtaga agatgcagta gctcattccg gcggcatgga cgagctgtac
     1621 aagtaagcgg ccgcgactct agatcataat cagccatacc acatt
//
