LOCUS       sequence-89066-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-ER-5
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        13..64
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     15..35
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      159..168
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             192..842
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 tccgccccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag
       61 agctggttta gtgaaccgtc agatccgcta gcgccaccat gctgctatcc gtgccgttgc
      121 tgctcggcct cctcggcctg gccgtcgccg caccggtcgc caccatggtg agcaagggcg
      181 aggagaataa catggccgtg attaaaccag agatgaagat gaggtactac atggacggct
      241 ccgtcaatgg gcatgagttc acaattgaag gtgaaggcac aggcagacct tacgagggac
      301 atcaagagat gacactacgc gtcacaatgg ccaagggcgg gccaatgcct ttcgcgtttg
      361 acttagtgtc acacgtgttc tgttacggcc acagaccttt tactaaatat ccagaagaga
      421 taccagacta tttcaaacaa gcatttcctg aaggcctgtc atgggaaagg tcgttggagt
      481 tcgaagatgg tgggtccgct tcagtcagtg cgcatataag ccttagagga aacaccttct
      541 accacaaatc caaatttact ggggttaact ttcctgccga tggtcctatc atgcaaaacc
      601 aaagtgttga ttgggagcca tcaaccgaga aaattactgc cagcgacgga gttctgaagg
      661 gtgatgttac gatgtaccta aaacttgaag gaggcggcaa tcacaaatgc caattcaaga
      721 ctacttacaa ggcggcaaaa aagattctta aaatgccagg aagccattac atcagccatc
      781 gcctcgtcag gaaaaccgaa ggcaacatta ctgagctggt agaagatgca gtagctcatt
      841 ccggcggcat ggacgagctg tacaagtccg gaaaggacga gctgtaagaa ttctgcagtc
      901 gacggtaccg cgggcccggg atccaccgga tctagataac tgatcataat cagccatacc
      961 acatttgtag aggttttact tgctttaaaa aaac
//
