LOCUS       sequence-89067-e        1768 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleoli, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1768)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..1768
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             62..712
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
     CDS             complement(1532..1543)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaataa
       61 catggccgtg attaaaccag agatgaagat gaggtactac atggacggct ccgtcaatgg
      121 gcatgagttc acaattgaag gtgaaggcac aggcagacct tacgagggac atcaagagat
      181 gacactacgc gtcacaatgg ccaagggcgg gccaatgcct ttcgcgtttg acttagtgtc
      241 acacgtgttc tgttacggcc acagaccttt tactaaatat ccagaagaga taccagacta
      301 tttcaaacaa gcatttcctg aaggcctgtc atgggaaagg tcgttggagt tcgaagatgg
      361 tgggtccgct tcagtcagtg cgcatataag ccttagagga aacaccttct accacaaatc
      421 caaatttact ggggttaact ttcctgccga tggtcctatc atgcaaaacc aaagtgttga
      481 ttgggagcca tcaaccgaga aaattactgc cagcgacgga gttctgaagg gtgatgttac
      541 gatgtaccta aaacttgaag gaggcggcaa tcacaaatgc caattcaaga ctacttacaa
      601 ggcggcaaaa aagattctta aaatgccagg aagccattac atcagccatc gcctcgtcag
      661 gaaaaccgaa ggcaacatta ctgagctggt agaagatgca gtagctcatt ccggcggcat
      721 ggacgagctg tacaagtccg gactcagatc tcgagccatg aagccaggat tcagtccccg
      781 tgggggtggc tttggcggcc gagggggctt tggtgaccgt ggtggtcgtg gaggccgagg
      841 gggctttggc gggggccgag gccaaggcgg aggctttaga ggtcgtggac gaggaggagg
      901 tggaggcggc ggcggcggtg gaggaggagg aagaggtggt ggaggcttcc attttggtgg
      961 caaccggggt cgtggtcggg gaggaaaaag aggaaaccag tcggggaaga atgtgatggt
     1021 ggagccgcat cggcatgagg gtgtcttcat ttgtcgggga aaggaagatg cactggtcac
     1081 caagaacctg gtccctgggg aatcagttta tggagagaag agagtctcga tttcggaagg
     1141 agatgacaaa attgagtacc gagcctggaa ccccttccgc tccaagcttg cagcagcaat
     1201 cctgggtggt gtggaccaga tccacatcaa accgggggct aaggttctct acctcggggc
     1261 tgcctcgggc accacggtct cccatgtctc tgacatcgtt ggtccggatg gtctagtcta
     1321 tgcagtcgag ttctcccacc gctctggccg tgacctcatt aacttggcca agaagaggac
     1381 caacatcatt cctgtgatcg aggatgctcg acacccacac aaataccgca tgctcatcgc
     1441 aatggtggat gtgatctttg ctgatgtggc ccagccagac cagacccgga ttgtggccct
     1501 gaatgcccac accttcctgc gtaatggagg acactttgtg atttccatta aggccaactg
     1561 cattgactcc acagcctcag ccgaggccgt gtttgcctcc gaagtgaaaa agatgcaaca
     1621 ggagaacatg aagccgcagg agcagttgac ccttgagcca tatgaaagag accatgccgt
     1681 ggtcgtggga gtgtacaggc caccccccaa ggtgaagaac tgaggatcca ccggatctag
     1741 ataactgatc ataatcagcc ataccaca
//
