LOCUS       sequence-89069-e        1135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi Complex, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1135)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-Golgi-7
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        9..60
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     11..31
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      350..359
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             383..1033
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct
       61 ggtttagtga accgtcagat ccgctagcat gaggcttcgg gagccgctcc tgagcggcag
      121 cgccgcgatg ccaggcgcgt ccctacagcg ggcctgccgc ctgctcgtgg ccgtctgcgc
      181 tctgcacctt ggcgtcaccc tcgtttacta cctggctggc cgcgacctga gccgcctgcc
      241 ccaactggtc ggagtctcca caccgctgca gggcggctcg aacagtgccg ccgccatcgg
      301 gcagtcctcc ggggagctcc ggaccggagg ggccaaggat ccaccggtcg ccaccatggt
      361 gagcaagggc gaggagaata acatggccgt gattaaacca gagatgaaga tgaggtacta
      421 catggacggc tccgtcaatg ggcatgagtt cacaattgaa ggtgaaggca caggcagacc
      481 ttacgaggga catcaagaga tgacactacg cgtcacaatg gccaagggcg ggccaatgcc
      541 tttcgcgttt gacttagtgt cacacgtgtt ctgttacggc cacagacctt ttactaaata
      601 tccagaagag ataccagact atttcaaaca agcatttcct gaaggcctgt catgggaaag
      661 gtcgttggag ttcgaagatg gtgggtccgc ttcagtcagt gcgcatataa gccttagagg
      721 aaacaccttc taccacaaat ccaaatttac tggggttaac tttcctgccg atggtcctat
      781 catgcaaaac caaagtgttg attgggagcc atcaaccgag aaaattactg ccagcgacgg
      841 agttctgaag ggtgatgtta cgatgtacct aaaacttgaa ggaggcggca atcacaaatg
      901 ccaattcaag actacttaca aggcggcaaa aaagattctt aaaatgccag gaagccatta
      961 catcagccat cgcctcgtca ggaaaaccga aggcaacatt actgagctgg tagaagatgc
     1021 agtagctcat tccggcggca tggacgagct gtacaagtaa gcggccgcga ctctagatca
     1081 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccaca
//
