LOCUS       sequence-89070-e        1378 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1378)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-H1-10
FEATURES             Location/Qualifiers
     source          1..1378
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      630..639
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             663..1313
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 cgtcagatcc gctagccgcc accatgaccg agaactccac ctccgccccg gcggcgaagc
       61 ccaaacgggc caaggcttcc aagaagtcca cggaccaccc caagtattca gacatgatcg
      121 tggctgctat ccaggcagag aagaaccgtg ccggctcctc gcgccagtcc atccaaaagt
      181 atatcaagag ccactacaag gtgggtgaga acgccgactc ccagatcaag ttgtccatca
      241 agcgcctagt gaccaccggt gttctcaagc aaaccaaagg ggtgggcgcc tcggggtcct
      301 tcaggctggc caagggcgat gagcccaaaa ggtcggtggc tttcaagaag accaagaagg
      361 aagtcaagaa agtggccact ccaaagaagg cagccaagcc caagaaggct gcctccaaag
      421 ccccaagcaa gaaacccaaa gccacccctg tcaagaaggc caagaagaag ccggctgcca
      481 cgcccaagaa agccaaaaag cccaaggttg tcaaagtcaa accagtcaag gcctccaaac
      541 ccaagaaggc caaaaccgtg aagcccaaag ccaagtcgag tgccaagagg gggagcaaga
      601 agaagtcggg aagcacggat ccaccggtcg ccaccatggt gagcaagggc gaggagaata
      661 acatggccgt gattaaacca gagatgaaga tgaggtacta catggacggc tccgtcaatg
      721 ggcatgagtt cacaattgaa ggtgaaggca caggcagacc ttacgaggga catcaagaga
      781 tgacactacg cgtcacaatg gccaagggcg ggccaatgcc tttcgcgttt gacttagtgt
      841 cacacgtgtt ctgttacggc cacagacctt ttactaaata tccagaagag ataccagact
      901 atttcaaaca agcatttcct gaaggcctgt catgggaaag gtcgttggag ttcgaagatg
      961 gtgggtccgc ttcagtcagt gcgcatataa gccttagagg aaacaccttc taccacaaat
     1021 ccaaatttac tggggttaac tttcctgccg atggtcctat catgcaaaac caaagtgttg
     1081 attgggagcc atcaaccgag aaaattactg ccagcgacgg agttctgaag ggtgatgtta
     1141 cgatgtacct aaaacttgaa ggaggcggca atcacaaatg ccaattcaag actacttaca
     1201 aggcggcaaa aaagattctt aaaatgccag gaagccatta catcagccat cgcctcgtca
     1261 ggaaaaccga aggcaacatt actgagctgg tagaagatgc agtagctcat tccggcggca
     1321 tggacgagct gtacaagtaa gcggccgcga ctctagatca taatcagcca taccacat
//
