LOCUS       sequence-89072-e        1218 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1218)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1218)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-H2B-6
FEATURES             Location/Qualifiers
     source          1..1218
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..456
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      469..478
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             502..1152
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgccaccat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc
      121 tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc
      181 aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc
      241 atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc
      301 gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag
      361 atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag
      421 ggtactaagg ccatcaccaa gtacaccagc gctaaggatc caccggtcgc caccatggtg
      481 agcaagggcg aggagaataa catggccgtg attaaaccag agatgaagat gaggtactac
      541 atggacggct ccgtcaatgg gcatgagttc acaattgaag gtgaaggcac aggcagacct
      601 tacgagggac atcaagagat gacactacgc gtcacaatgg ccaagggcgg gccaatgcct
      661 ttcgcgtttg acttagtgtc acacgtgttc tgttacggcc acagaccttt tactaaatat
      721 ccagaagaga taccagacta tttcaaacaa gcatttcctg aaggcctgtc atgggaaagg
      781 tcgttggagt tcgaagatgg tgggtccgct tcagtcagtg cgcatataag ccttagagga
      841 aacaccttct accacaaatc caaatttact ggggttaact ttcctgccga tggtcctatc
      901 atgcaaaacc aaagtgttga ttgggagcca tcaaccgaga aaattactgc cagcgacgga
      961 gttctgaagg gtgatgttac gatgtaccta aaacttgaag gaggcggcaa tcacaaatgc
     1021 caattcaaga ctacttacaa ggcggcaaaa aagattctta aaatgccagg aagccattac
     1081 atcagccatc gcctcgtcag gaaaaccgaa ggcaacatta ctgagctggt agaagatgca
     1141 gtagctcatt ccggcggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat
     1201 aatcagccat accacatt
//
