LOCUS       sequence-89073-e        1247 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1247)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-H3-23
FEATURES             Location/Qualifiers
     source          1..1247
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    437..489
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      497..506
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             530..1180
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagccg ccaccatggc tcgtactaaa cagacagctc ggaaatccac
       61 cggcggtaaa gcgccacgca agcagctggc taccaaggct gctcgcaaga gcgcgccggc
      121 taccggcggc gtgaaaaagc ctcaccgtta ccgcccgggc actgtggctc tgcgcgagat
      181 ccgccgctac caaaagtcga ccgagttgct gattcggaag ctgccgttcc agcgcctggt
      241 gcgagaaatc gcccaagact tcaagaccga tcttcgcttc cagagctctg cggtgatggc
      301 gctgcaggag gcttgtgagg cctacttggt agggctcttt gaggacacaa acctttgcgc
      361 catccatgct aagcgagtga ctattatgcc caaagacatc cagctcgctc gccgcattcg
      421 cggagaaaga gcggatctcg agctcaagct tcgaattctg cagtcgacgg taccgcgggc
      481 ccgggatcca ccggtcgcca ccatggtgag caagggcgag gagaataaca tggccgtgat
      541 taaaccagag atgaagatga ggtactacat ggacggctcc gtcaatgggc atgagttcac
      601 aattgaaggt gaaggcacag gcagacctta cgagggacat caagagatga cactacgcgt
      661 cacaatggcc aagggcgggc caatgccttt cgcgtttgac ttagtgtcac acgtgttctg
      721 ttacggccac agacctttta ctaaatatcc agaagagata ccagactatt tcaaacaagc
      781 atttcctgaa ggcctgtcat gggaaaggtc gttggagttc gaagatggtg ggtccgcttc
      841 agtcagtgcg catataagcc ttagaggaaa caccttctac cacaaatcca aatttactgg
      901 ggttaacttt cctgccgatg gtcctatcat gcaaaaccaa agtgttgatt gggagccatc
      961 aaccgagaaa attactgcca gcgacggagt tctgaagggt gatgttacga tgtacctaaa
     1021 acttgaagga ggcggcaatc acaaatgcca attcaagact acttacaagg cggcaaaaaa
     1081 gattcttaaa atgccaggaa gccattacat cagccatcgc ctcgtcagga aaaccgaagg
     1141 caacattact gagctggtag aagatgcagt agctcattcc ggcggcatgg acgagctgta
     1201 caagtaagcg gccgcgactc tagatcataa tcagccatac cacattg
//
