LOCUS       sequence-89074-e        1147 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1147)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1147)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-H4-23
FEATURES             Location/Qualifiers
     source          1..1147
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    338..390
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      398..407
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             431..1081
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagccg ccaccatgtc tggccgcggc aaaggcggga agggtcttgg
       61 caaaggcggc gctaagcgcc accgtaaagt actgcgcgac aatatccagg gcatcaccaa
      121 gccggccatc cggcgccttg ctcgccgcgg cggcgtgaag cgcatctccg gcctcatcta
      181 cgaggagact cgcggggtgc tgaaggtgtt cctggagaac gtgatccggg acgccgtgac
      241 ctatacagag cacgccaagc gcaagacggt caccgccatg gatgtggtct acgcgctcaa
      301 gcgccagggc cgcaccctct acggtttcgg tggggatctc gagctcaagc ttcgaattct
      361 gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtga gcaagggcga
      421 ggagaataac atggccgtga ttaaaccaga gatgaagatg aggtactaca tggacggctc
      481 cgtcaatggg catgagttca caattgaagg tgaaggcaca ggcagacctt acgagggaca
      541 tcaagagatg acactacgcg tcacaatggc caagggcggg ccaatgcctt tcgcgtttga
      601 cttagtgtca cacgtgttct gttacggcca cagacctttt actaaatatc cagaagagat
      661 accagactat ttcaaacaag catttcctga aggcctgtca tgggaaaggt cgttggagtt
      721 cgaagatggt gggtccgctt cagtcagtgc gcatataagc cttagaggaa acaccttcta
      781 ccacaaatcc aaatttactg gggttaactt tcctgccgat ggtcctatca tgcaaaacca
      841 aagtgttgat tgggagccat caaccgagaa aattactgcc agcgacggag ttctgaaggg
      901 tgatgttacg atgtacctaa aacttgaagg aggcggcaat cacaaatgcc aattcaagac
      961 tacttacaag gcggcaaaaa agattcttaa aatgccagga agccattaca tcagccatcg
     1021 cctcgtcagg aaaaccgaag gcaacattac tgagctggta gaagatgcag tagctcattc
     1081 cggcggcatg gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata
     1141 ccacatt
//
