LOCUS       sequence-89076-e        2549 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2549)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..2549
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             61..711
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagaataac
       61 atggccgtga ttaaaccaga gatgaagatg aggtactaca tggacggctc cgtcaatggg
      121 catgagttca caattgaagg tgaaggcaca ggcagacctt acgagggaca tcaagagatg
      181 acactacgcg tcacaatggc caagggcggg ccaatgcctt tcgcgtttga cttagtgtca
      241 cacgtgttct gttacggcca cagacctttt actaaatatc cagaagagat accagactat
      301 ttcaaacaag catttcctga aggcctgtca tgggaaaggt cgttggagtt cgaagatggt
      361 gggtccgctt cagtcagtgc gcatataagc cttagaggaa acaccttcta ccacaaatcc
      421 aaatttactg gggttaactt tcctgccgat ggtcctatca tgcaaaacca aagtgttgat
      481 tgggagccat caaccgagaa aattactgcc agcgacggag ttctgaaggg tgatgttacg
      541 atgtacctaa aacttgaagg aggcggcaat cacaaatgcc aattcaagac tacttacaag
      601 gcggcaaaaa agattcttaa aatgccagga agccattaca tcagccatcg cctcgtcagg
      661 aaaaccgaag gcaacattac tgagctggta gaagatgcag tagctcattc cggcggcatg
      721 gacgagctgt acaagtccgg actcagatct cgagctcaag cttcgatggc gactgcgacc
      781 cccgtgccgc cgcggatggg cagccgcgct ggcggcccca ccacgccgct gagccccacg
      841 cgcctgtcgc ggctccagga gaaggaggag ctgcgcgagc tcaatgaccg gctggcggtg
      901 tacatcgaca aggtgcgcag cctggagacg gagaacagcg cgctgcagct gcaggtgacg
      961 gagcgcgagg aggtgcgcgg ccgtgagctc accggcctca aggcgctcta cgagaccgag
     1021 ctggccgacg cgcgacgcgc gctcgacgac acggcccgcg agcgcgccaa gctgcagatc
     1081 gagctgggca agtgcaaggc ggaacacgac cagctgctcc tcaactatgc taagaaggaa
     1141 tctgatctta atggcgccca gatcaagctt cgagaatatg aagcagcact caattcgaaa
     1201 gatgcagctc ttgctactgc acttggtgac aaaaaaagtt tagagggaga tttggaggat
     1261 ctgaaggatc agattgccca gttggaagcc tccttagctg cagccaaaaa acagttagca
     1321 gatgaaactt tacttaaagt agatttggag aatcgttgtc agagccttac tgaggacttg
     1381 gagtttcgca aaagcatgta tgaagaggag attaacgaga ccagaaggaa gcatgaaacg
     1441 cgcttggtag aggtggattc tgggcgtcaa attgagtatg agtacaagct ggcgcaagcc
     1501 cttcatgaga tgagagagca acatgatgcc caagtgaggc tgtataagga ggagctggag
     1561 cagacttacc atgccaaact tgagaatgcc agactgtcat cagagatgaa tacttctact
     1621 gtcaacagtg ccagggaaga actgatggaa agccgcatga gaattgagag cctttcatcc
     1681 cagctttcta atctacagaa agagtctaga gcatgtttgg aaaggattca agaattagag
     1741 gacttgcttg ctaaagaaaa agacaactct cgtcgcatgc tgacagacaa agagagagag
     1801 atggcggaaa taagggatca aatgcagcaa cagctgaatg actatgaaca gcttcttgat
     1861 gtaaagttag ccctggacat ggaaatcagt gcttacagga aactcttaga aggcgaagaa
     1921 gagaggttga agctgtctcc aagcccttct tcccgtgtga cagtatcccg agcatcctca
     1981 agtcgtagtg tacgtacaac tagaggaaag cggaagaggg ttgatgtgga agaatcagag
     2041 gcgagtagta gtgttagcat ctctcattcc gcctcagcca ctggaaatgt ttgcatcgaa
     2101 gaaattgatg ttgatgggaa atttatccgc ttgaagaaca cttctgaaca ggatcaacca
     2161 atgggaggct gggagatgat cagaaaaatt ggagacacat cagtcagtta taaatatacc
     2221 tcaagatatg tgctgaaggc aggccagact gttacaattt gggctgcaaa cgctggtgtc
     2281 acagccagcc ccccaactga cctcatctgg aagaaccaga actcgtgggg cactggcgaa
     2341 gatgtgaagg ttatattgaa aaattctcag ggagaggagg ttgctcaaag aagtacagtc
     2401 tttaaaacaa ccatacctga agaagaggag gaggaggaag aagcagctgg agtggttgtt
     2461 gaggaagaac ttttccacca gcagggaacc ccaagagcat ccaatagaag ctgtgcaatt
     2521 atgtagggat ccaccggatc tagataact
//
