LOCUS       sequence-89078-e        2059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Lysosome Membrane, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2059)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-Lysosomes-20
FEATURES             Location/Qualifiers
     source          1..2059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      35..44
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      39..48
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(41..103,104..1261)
                     /codon_start=1
                     /product="rat lysosome-associated membrane glycoprotein 1"
                     /label=LAMP1
                     /translation="MAAPGARRPLLLLLLAGLAHSAPALFEVKDNNGTACIMASFSASF
                     LTTYEAGHVSKVSNMTLPASAEVLKNSSSCGEKNASEPTLAITFGEGYLLKLTFTKNTT
                     RYSVQHMYFTYNLSDTQFFPNASSKGPDTVDSTTDIKADINKTYRCVSDIRVYMKNVTI
                     VLWDATIQAYLPSSNFSKEETRCPQDQPSPTTGPPSPSPPLVPTNPSVSKYNVTGDNGT
                     CLLASMALQLNITYMKKDNTTVTRAFNINPSDKYSGTCGAQLVTLKVGNKSRVLELQFG
                     MNATSSLFFLQGVQLNMTLPDAIEPTFSTSNYSLKALQASVGNSYKCNSEEHIFVSKAL
                     ALNVFSVQVQAFRVESDRFGSVEECVQDGNNMLIPIAVGGALAGLVLIVLIAYLIGRKR
                     SHAGYQTI"
     regulatory      1316..1325
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1349..1999
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 gatccgctag cgctaccgga ctcagatctc gagcgccacc atggcggccc cgggcgcccg
       61 gcgtccgctg ctcctgttgc tgctggcagg ccttgcacac agcgccccag cactgttcga
      121 ggtgaaagac aacaacggca cagcgtgtat aatggccagc ttctctgcct cctttctgac
      181 cacctatgag gctggacatg tttctaaggt ctcgaatatg accctgccag cctctgcaga
      241 agtcctgaag aatagcagct cttgtggtga aaagaatgct tctgagccca ccctcgcaat
      301 cacctttgga gaaggatatt tactgaaact caccttcaca aaaaacacaa cacgttacag
      361 tgtccagcac atgtatttca catataacct gtcagacaca caattctttc ccaatgccag
      421 ctccaaaggg cccgacactg tggattccac aactgacatc aaggcagaca tcaacaaaac
      481 ataccgatgt gtcagcgaca tcagggtcta catgaagaat gtgaccattg tgctctggga
      541 cgctactatc caggcctacc tgccgagtag caacttcagc aaggaagaga cacgctgccc
      601 acaggatcaa ccttccccaa ctactgggcc acccagcccc tcaccaccac ttgtgcccac
      661 aaaccccagt gtgtccaagt acaatgtgac tggtgacaat ggaacctgcc tgctggcctc
      721 tatggcactg caactcaaca tcacctacat gaagaaggac aacacgactg tgaccagagc
      781 attcaacatc aacccaagtg acaaatatag tgggacttgc ggtgcccagt tggtgaccct
      841 gaaggtgggg aacaagagca gagtcctgga gctgcagttt gggatgaatg ccacttctag
      901 cctgtttttc ctgcaaggag ttcagttgaa catgactctt cctgatgcca tagagcccac
      961 gttcagcacc tccaactatt ccctgaaagc tcttcaggcc agtgtcggca actcatacaa
     1021 gtgcaacagt gaggagcaca tctttgtcag caaggcgctc gccctcaatg tcttcagcgt
     1081 gcaagtccag gctttcaggg tagaaagtga caggtttggg tctgtggaag agtgtgtaca
     1141 ggacggtaac aacatgctga tccccattgc tgtgggcggg gccctggcag ggctggtcct
     1201 catcgtcctc atcgcctacc tcatcggcag gaagaggagt cacgcgggct atcagaccat
     1261 ctcggaattc ggctccaccg gctccaccgg ctccaccggc gcggatccac cggtcgccac
     1321 catggtgagc aagggcgagg agaataacat ggccgtgatt aaaccagaga tgaagatgag
     1381 gtactacatg gacggctccg tcaatgggca tgagttcaca attgaaggtg aaggcacagg
     1441 cagaccttac gagggacatc aagagatgac actacgcgtc acaatggcca agggcgggcc
     1501 aatgcctttc gcgtttgact tagtgtcaca cgtgttctgt tacggccaca gaccttttac
     1561 taaatatcca gaagagatac cagactattt caaacaagca tttcctgaag gcctgtcatg
     1621 ggaaaggtcg ttggagttcg aagatggtgg gtccgcttca gtcagtgcgc atataagcct
     1681 tagaggaaac accttctacc acaaatccaa atttactggg gttaactttc ctgccgatgg
     1741 tcctatcatg caaaaccaaa gtgttgattg ggagccatca accgagaaaa ttactgccag
     1801 cgacggagtt ctgaagggtg atgttacgat gtacctaaaa cttgaaggag gcggcaatca
     1861 caaatgccaa ttcaagacta cttacaaggc ggcaaaaaag attcttaaaa tgccaggaag
     1921 ccattacatc agccatcgcc tcgtcaggaa aaccgaaggc aacattactg agctggtaga
     1981 agatgcagta gctcattccg gcggcatgga cgagctgtac aagtaagcgg ccgcgactct
     2041 agatcataat cagccatac
//
