LOCUS       sequence-89079-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-Mito-7
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..48
                     /label=CMVd3 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields strongly reduced expression 
                     relative to the full-length promoter"
     CDS             77..151
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      179..188
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             212..862
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg tttagtgaac
       61 cgtcagatcc gctagcatgt ccgtcctgac gccgctgctg ctgcggggct tgacaggctc
      121 ggcccggcgg ctcccagtgc cgcgcgccaa gatccattcg ttgggggatc caccggtcgc
      181 caccatggtg agcaagggcg aggagaataa catggccgtg attaaaccag agatgaagat
      241 gaggtactac atggacggct ccgtcaatgg gcatgagttc acaattgaag gtgaaggcac
      301 aggcagacct tacgagggac atcaagagat gacactacgc gtcacaatgg ccaagggcgg
      361 gccaatgcct ttcgcgtttg acttagtgtc acacgtgttc tgttacggcc acagaccttt
      421 tactaaatat ccagaagaga taccagacta tttcaaacaa gcatttcctg aaggcctgtc
      481 atgggaaagg tcgttggagt tcgaagatgg tgggtccgct tcagtcagtg cgcatataag
      541 ccttagagga aacaccttct accacaaatc caaatttact ggggttaact ttcctgccga
      601 tggtcctatc atgcaaaacc aaagtgttga ttgggagcca tcaaccgaga aaattactgc
      661 cagcgacgga gttctgaagg gtgatgttac gatgtaccta aaacttgaag gaggcggcaa
      721 tcacaaatgc caattcaaga ctacttacaa ggcggcaaaa aagattctta aaatgccagg
      781 aagccattac atcagccatc gcctcgtcag gaaaaccgaa ggcaacatta ctgagctggt
      841 agaagatgca gtagctcatt ccggcggcat ggacgagctg tacaagtaag cggccgcgac
      901 tctagatcat aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc
      961 cacacctccc cctgaacctg aaacataaaa tgaa
//
