LOCUS       sequence-89084-e        2130 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Microtubules, Excitation: 548, Emission: 561.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2130)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mKOet-Tubulin-6
FEATURES             Location/Qualifiers
     source          1..2130
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             62..712
                     /codon_start=1
                     /product="monomeric Kusabira-Orange fluorescent protein 
                     (Karasawa et al., 2004)"
                     /label=mKO
                     /translation="MAVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRVT
                     MAKGGPMPFAFDLVSHVFCYGHRPFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSAS
                     VSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMYL
                     KLEGGGNHKCQFKTTYKAAKKILKMPGSHYISHRLVRKTEGNITELVEDAVAHS"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaataa
       61 catggccgtg attaaaccag agatgaagat gaggtactac atggacggct ccgtcaatgg
      121 gcatgagttc acaattgaag gtgaaggcac aggcagacct tacgagggac atcaagagat
      181 gacactacgc gtcacaatgg ccaagggcgg gccaatgcct ttcgcgtttg acttagtgtc
      241 acacgtgttc tgttacggcc acagaccttt tactaaatat ccagaagaga taccagacta
      301 tttcaaacaa gcatttcctg aaggcctgtc atgggaaagg tcgttggagt tcgaagatgg
      361 tgggtccgct tcagtcagtg cgcatataag ccttagagga aacaccttct accacaaatc
      421 caaatttact ggggttaact ttcctgccga tggtcctatc atgcaaaacc aaagtgttga
      481 ttgggagcca tcaaccgaga aaattactgc cagcgacgga gttctgaagg gtgatgttac
      541 gatgtaccta aaacttgaag gaggcggcaa tcacaaatgc caattcaaga ctacttacaa
      601 ggcggcaaaa aagattctta aaatgccagg aagccattac atcagccatc gcctcgtcag
      661 gaaaaccgaa ggcaacatta ctgagctggt agaagatgca gtagctcatt ccggcggcat
      721 ggacgagctg tacaagtccg gactcagatc tcgagtgcgt gagtgcatct ccatccacgt
      781 tggccaggct ggtgtccaga ttggcaatgc ctgctgggag ctctactgcc tggaacacgg
      841 catccagccc gatggccaga tgccaagtga caagaccatt gggggaggag atgactcctt
      901 caacaccttc ttcagtgaga cgggcgctgg caagcacgtg ccccgggctg tgtttgtaga
      961 cttggaaccc acagtcattg atgaagttcg cactggcacc taccgccagc tcttccaccc
     1021 tgagcagctc atcacaggca aggaagatgc tgccaataac tatgcccgag ggcactacac
     1081 cattggcaag gagatcattg accttgtgtt ggaccgaatt cgcaagctgg ctgaccagtg
     1141 caccggtctt cagggcttct tggttttcca cagctttggt gggggaactg gttctgggtt
     1201 cacctccctg ctcatggaac gtctctcagt tgattatggc aagaagtcca agctggagtt
     1261 ctccatttac ccagcacccc aggtttccac agctgtagtt gagccctaca actccatcct
     1321 caccacccac accaccctgg agcactctga ttgtgccttc atggtagaca atgaggccat
     1381 ctatgacatc tgtcgtagaa acctcgatat cgagcgccca acctacacta accttaaccg
     1441 ccttattagc cagattgtgt cctccatcac tgcttccctg agatttgatg gagccctgaa
     1501 tgttgacctg acagaattcc agaccaacct ggtgccctac ccccgcatcc acttccctct
     1561 ggccacatat gcccctgtca tctctgctga gaaagcctac catgaacagc tttctgtagc
     1621 agagatcacc aatgcttgct ttgagccagc caaccagatg gtgaaatgtg accctcgcca
     1681 tggtaaatac atggcttgct gcctgttgta ccgtggtgac gtggttccca aagatgtcaa
     1741 tgctgccatt gccaccatca aaaccaagcg cagcatccag tttgtggatt ggtgccccac
     1801 tggcttcaag gttggcatca actaccagcc tcccactgtg gtgcctggtg gagacctggc
     1861 caaggtacag agagctgtgt gcatgctgag caacaccaca gccattgctg aggcctgggc
     1921 tcgcctggac cacaagtttg acctgatgta tgccaagcgt gcctttgttc actggtacgt
     1981 gggtgagggg atggaggaag gcgagttttc agaggcccgt gaagatatgg ctgcccttga
     2041 gaaggattat gaggaggttg gtgtggattc tgttgaagga gagggtgagg aagaaggaga
     2101 ggaatactaa ggatccaccg gatctagata
//
