LOCUS       sequence-89089-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 548, Emission: 562.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mOrange-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      259..268
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             265..975
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     678..697
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aggcgtgtac ggtgggaggt ctatataagc agagctggtt tagtgaaccc gtcagatccg
       61 ctagcgctac cggactcaga tctggtaccc cttgcagctt ccagttgtca ggaagagaag
      121 gctcccatgg atcctgtggt ggtgctgggg ctctgtctct cctgtttgct tctcctttca
      181 ctctggaaac agagctatgg gggagggaag cttcgaattc tgcagtcgac ggtaccgcgg
      241 gcccgggatc caccggtcgc caccatggtg agcaagggcg aggagaataa catggccatc
      301 atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc
      361 gagatcgagg gcgagggcga gggccgcccc tacgagggct ttcagaccgc taagctgaag
      421 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcacctac
      481 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttcaa gctgtccttc
      541 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
      601 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
      661 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
      721 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagatgag gctgaagctg
      781 aaggacggcg gccactacac ctccgaggtc aagaccacct acaaggccaa gaagcccgtg
      841 cagctgcccg gcgcctacat cgtcggcatc aagttggaca tcacctccca caacgaggac
      901 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
      961 gagctgtaca agtaagcggc cgcgactcta gatcataatc agccatacca catttgtaga
     1021 ggttttactt gctttaaaaa acctcccaca cctccccctg acctg
//
