LOCUS       sequence-89090-e        1223 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 548, Emission: 562.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW
  TITLE     Photoconversion in orange and red fluorescent proteins.
  JOURNAL   Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009
            Apr 12.
  PUBMED    19363494
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1319"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "355-8"
COMMENT     Sequence Label: mOrange-H2B-6
FEATURES             Location/Qualifiers
     source          1..1223
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..455
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      468..477
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             474..1184
                     /codon_start=1
                     /product="orange monomeric derivative of DsRed fluorescent 
                     protein (Shanet et al., 2004)"
                     /label=mOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVGIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     887..906
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattctgcag
       61 tcgacggtac cgccaccatg ccagagccag cgaagtctgc tcccgccccg aaaaagggct
      121 ccaagaaggc ggtgactaag gcgcagaaga aaggcggcaa gaagcgcaag cgcagccgca
      181 aggagagcta ttccatctat gtgtacaagg ttctgaagca ggtccaccct gacaccggca
      241 tttcgtccaa ggccatgggc atcatgaatt cgtttgtgaa cgacattttc gagcgcatcg
      301 caggtgaggc ttcccgcctg gcgcattaca acaagcgctc gaccatcacc tccagggaga
      361 tccagacggc cgtgcgcctg ctgctgcctg gggagttggc caagcacgcc gtgtccgagg
      421 gtactaaggc catcaccaag tacaccagcg ctaaggatcc accggtcgcc accatggtga
      481 gcaagggcga ggagaataac atggccatca tcaaggagtt catgcgcttc aaggtgcgca
      541 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
      601 acgagggctt tcagaccgct aagctgaagg tgaccaaggg tggccccctg cccttcgcct
      661 gggacatcct gtcccctcag ttcacctacg gctccaaggc ctacgtgaag caccccgccg
      721 acatccccga ctacttcaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
      781 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
      841 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
      901 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
      961 agggcgagat caagatgagg ctgaagctga aggacggcgg ccactacacc tccgaggtca
     1021 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacatc gtcggcatca
     1081 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     1141 agggccgcca ctccaccggc ggcatggacg agctgtacaa gtaagcggcc gcgactctag
     1201 atcataatca gccataccac att
//
