LOCUS       sequence-89095-e        1470 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Clathrin Vesicles, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1470)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 1470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mOrange2-Clathrin-15
FEATURES             Location/Qualifiers
     source          1..1470
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..742
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 tgacgtcaga tccgctagcg ctaccggtcg ccccatggtg agcaagggcg aggagaataa
       61 catggccatc atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg
      121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggct ttcagaccgc
      181 taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      241 tttcacctac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttcaa
      301 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aactacgagg acggcggcgt
      361 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtgatgcag aagaagacca tgggctggga
      481 ggcctcctcc gagcggatgt accccgagga cggtgccctg aagggcaaga tcaagatgag
      541 gctgaagctg aaggacggcg gccactacac ctccgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcacctccca
      661 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggact cagatctcga gctcaagctt cgaattctgc
      781 agtcgacatg gctgatgact ttggcttctt ctcgtcgtcg gagagtggtg ccccggaggc
      841 ggcggaggag gacccggcgg ccgccttcct ggcccagcag gagagcgaga ttgcaggcat
      901 agagaacgac gagggcttcg gggcacctgc cggcagccat gcggcccccg cacagccggg
      961 ccccacgagt ggggctggtt ctgaggacat ggggaccaca gtcaatggag atgtgtttca
     1021 ggaggccaac ggtcctgctg atggctacgc agccattgcc caggctgaca ggctgaccca
     1081 ggagcctgag agcatccgca agtggcgaga ggagcagagg aaacggctgc aagagctgga
     1141 tgctgcatct aaggtcacgg aacaggaatg gcgggagaag gccaagaagg acctggagga
     1201 gtggaaccag cgccagagtg aacaagtaga gaagaacaag atcaacaacc gggcatccga
     1261 ggaggctttc gtgaaggaat ccaaggagga gaccccaggc acagagtggg agaaggtggc
     1321 ccagctatgt gacttcaacc ccaagagcag caagcagtgc aaagatgtgt cccgcctgcg
     1381 ctcggtgctc atgtccctga agcagacgcc actgtcccgc tagggatcca ccggatctag
     1441 ataactgatc ataatcagcc ataccacatt
//
