LOCUS       sequence-89097-e        1548 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mOrange2-Cx26-7
FEATURES             Location/Qualifiers
     source          1..1548
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      793..802
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             799..1509
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 cagatccgct agcgctaccg gactcagatc tcgagctcaa gcttcgaatt cgcggccgca
       61 cgctcctcgg gacacagtgc caaccatcca gaggacaaga tggattgggg cacactacag
      121 agcatcctcg ggggtgtcaa caagcactcc accagcattg ggaaaatctg gctcactgtc
      181 ctcttcatct tccgcatcat gatcctcgtg gtggccgcga aggaggtgtg gggagatgag
      241 caagccgatt ttgtttgcaa cactctccag cctggctgta agaatgtgtg ctacgaccac
      301 tacttcccca tctctcacat ccggctctgg gctctgcagc tgatcatggt gtccacgccg
      361 gccctcctgg tagctatgca cgtggcctac cggagacacg aaaagaaacg gaagttcatg
      421 aagggagaga taaagaacga gtttaaggac atcgaagaga tcaaaaccca gaaggtccgt
      481 atcgaagggt ccctgtggtg gacctacacc accagcatct tcttccgggt catcttcgaa
      541 gctgtcttca tgtatgtctt ttacatcatg tacaatggct tcttcatgca gcgtctggtg
      601 aagtgtaacg cctggccttg tcccaataca gtggactgct tcatttccag gcccacagaa
      661 aagactgtct tcacggtgtt catgatctct gtgtctggaa tttgcatcct gctaaacatc
      721 acagagctgt gctatctgtt cattaggtat tgctcaggga agtccaaaag accagtcgcg
      781 gatccaccgg tcgccaccat ggtgagcaag ggcgaggaga ataacatggc catcatcaag
      841 gagttcatgc gcttcaaggt gcgcatggag ggctccgtga acggccacga gttcgagatc
      901 gagggcgagg gcgagggccg cccctacgag ggctttcaga ccgctaagct gaaggtgacc
      961 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcatttcac ctacggctcc
     1021 aaggcctacg tgaagcaccc cgccgacatc cccgactact tcaagctgtc cttccccgag
     1081 ggcttcaagt gggagcgcgt gatgaactac gaggacggcg gcgtggtgac cgtgacccag
     1141 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     1201 ccctccgacg gccccgtgat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     1261 atgtaccccg aggacggtgc cctgaagggc aagatcaaga tgaggctgaa gctgaaggac
     1321 ggcggccact acacctccga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     1381 cccggcgcct acatcgtcga catcaagttg gacatcacct cccacaacga ggactacacc
     1441 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     1501 tacaagtaag cggccgcgac tctagatcat aatcagccat accacatt
//
