LOCUS       sequence-89100-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mOrange2-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      221..230
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             227..937
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 agagctgttt agtgacgtca gatccgctag cgctacggac tcagatctgg taccctgcag
       61 cttccagtgt caggaagaga aggctcccat ggatcctgtg gtggtgctgg ggctctgtct
      121 ctcctgtttg cttctccttt cactctggaa acagagctat gggggaggga agcttcgaat
      181 tctgcagtcg acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcaaggg
      241 cgaggagaat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg
      301 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
      361 ctttcagacc gctaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
      421 cctgtcccct catttcacct acggctccaa ggcctacgtg aagcaccccg ccgacatccc
      481 cgactacttc aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaactacga
      541 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
      601 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtgatgc agaagaagac
      661 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggtgccc tgaagggcaa
      721 gatcaagatg aggctgaagc tgaaggacgg cggccactac acctccgagg tcaagaccac
      781 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac atcgtcgaca tcaagttgga
      841 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
      901 ccactccacc ggcggcatgg acgagctgta caagtaagcg gccgcgactc tagatcataa
      961 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctcccct
     1021 gaactgaaac ataaaatgaa tgcatgtgtg tactgttatt gcagc
//
