LOCUS       sequence-89101-e        1664 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: MT End Binding Protein, Excitation: 549, Emission: 
            565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mOrange2-EB3-7
FEATURES             Location/Qualifiers
     source          1..1664
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      44..53
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      908..917
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             914..1624
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 ggactcagat ctcgagctca agcttcgaat tctgcagtcg actgccacca tggccgtcaa
       61 tgtgtactcc acatctgtga ccagtgaaaa tctgagtcgc catgatatgc ttgcatgggt
      121 caacgactcc ctgcacctca actataccaa gatagaacag ctttgttcag gggcagccta
      181 ctgccagttc atggacatgc tcttccccgg ctgtgtgcac ttgaggaaag tgaagttcca
      241 ggccaaacta gagcatgaat acatccacaa cttcaaggtg ctgcaagcag ctttcaagaa
      301 gatgggtgtt gacaaaatca ttcctgtaga gaaattagtg aaaggaaaat tccaagataa
      361 ttttgagttt attcagtggt ttaagaaatt ctttgacgca aactatgatg gaaaggatta
      421 caaccctctg ctggcgcggc agggccagga cgtagcgcca cctcctaacc caggtgatca
      481 gatcttcaac aaatccaaga aactcattgg cacagcagtt ccacagagga cgtcccccac
      541 aggcccaaaa aacatgcaga cctctggccg gctgagcaat gtggcccccc cctgcattct
      601 ccggaagaat cctccatcag cccgaaatgg cggccatgag actgatgccc aaattcttga
      661 actcaaccaa cagctggtgg acttgaagct gacagtggat gggctggaga aggaacgtga
      721 cttctacttc agcaaacttc gtgacatcga gctcatctgc caggagcatg aaagtgaaaa
      781 cagccctgtt atctcaggca tcattggcat cctctatgcc acagaggaag gattcgcacc
      841 ccctgaggac gatgagattg aagagcatca acaagaagac caggacgagt acctggatcc
      901 accggtcgcc accatggtga gcaagggcga ggagaataac atggccatca tcaaggagtt
      961 catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
     1021 cgagggcgag ggccgcccct acgagggctt tcagaccgct aagctgaagg tgaccaaggg
     1081 tggccccctg cccttcgcct gggacatcct gtcccctcat ttcacctacg gctccaaggc
     1141 ctacgtgaag caccccgccg acatccccga ctacttcaag ctgtccttcc ccgagggctt
     1201 caagtgggag cgcgtgatga actacgagga cggcggcgtg gtgaccgtga cccaggactc
     1261 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
     1321 cgacggcccc gtgatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta
     1381 ccccgaggac ggtgccctga agggcaagat caagatgagg ctgaagctga aggacggcgg
     1441 ccactacacc tccgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg
     1501 cgcctacatc gtcgacatca agttggacat cacctcccac aacgaggact acaccatcgt
     1561 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa
     1621 gtaagcggcc gcgactctag atcataatca gccataccac attg
//
