LOCUS       sequence-89102-e        1491 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi/ER, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1491)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mOrange2-ELP-1-25
FEATURES             Location/Qualifiers
     source          1..1491
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      22..31
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      739..748
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             745..1455
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc cgccaccatg gccatgaaca ttttccggct gactggggac
       61 ctgtcccacc tggcggccat cgtcatcctg ctgctgaaga tctggaagac gcgctcctgc
      121 gccggtattt ctgggaaaag ccagcttctg tttgcactgg tcttcacaac tcgttacctg
      181 gatcttttta cttcatttat ttcattgtat aacacatcta tgaaggttat ctaccttgcc
      241 tgctcctatg ccacagtgta cctgatctac ctgaaattta aggcaaccta cgatggaaat
      301 catgatacct tccgagtgga gtttctggtg gtccctgtgg gaggcctctc atttttagtt
      361 aatcacgatt tctctcctct tgagatcctc tggaccttct ccatctacct ggagtccgtg
      421 gctatccttc cgcagctgtt tatgatcagc aagactgggg aggccgagac catcaccacc
      481 cactacctgt tcttcctggg cctctatcgt gctttgtatc ttgtcaactg gatctggcgc
      541 ttctactttg agggcttctt tgacctcatt gctgtggtgg ccggcgtagt ccagaccatc
      601 ctatactgtg acttcttcta cttgtacatt acaaaagtac tcaagggaaa gaagctcagt
      661 ttgccagcag gcggtggggg ggagctcaag cttcgaattc tgcagtcgac ggtaccgcgg
      721 gcccgggatc caccggtcgc caccatggtg agcaagggcg aggagaataa catggccatc
      781 atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc
      841 gagatcgagg gcgagggcga gggccgcccc tacgagggct ttcagaccgc taagctgaag
      901 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca tttcacctac
      961 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttcaa gctgtccttc
     1021 cccgagggct tcaagtggga gcgcgtgatg aactacgagg acggcggcgt ggtgaccgtg
     1081 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     1141 aacttcccct ccgacggccc cgtgatgcag aagaagacca tgggctggga ggcctcctcc
     1201 gagcggatgt accccgagga cggtgccctg aagggcaaga tcaagatgag gctgaagctg
     1261 aaggacggcg gccactacac ctccgaggtc aagaccacct acaaggccaa gaagcccgtg
     1321 cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcacctccca caacgaggac
     1381 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     1441 gagctgtaca agtaagcggc cgcgactcta gatcataatc agccatacca c
//
