LOCUS       sequence-89103-e        1484 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endosomes, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1484)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 1484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mOrange2-Endo-14
FEATURES             Location/Qualifiers
     source          1..1484
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      33..42
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             39..746
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             792..821
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctgtgacgtc agatccgcta gcgctaccgg tcgccaccat ggtgagcaag ggcgaggaga
       61 ataacatggc catcatcaag gagttcatgc gcttcaaggt gcgcatggag ggctccgtga
      121 acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag ggctttcaga
      181 ccgctaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac atcctgtccc
      241 ctcatttcac ctacggctcc aaggcctacg tgaagcaccc cgccgacatc cccgactact
      301 tcaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaactac gaggacggcg
      361 gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc tacaaggtga
      421 agctgcgcgg caccaacttc ccctccgacg gccccgtgat gcagaagaag accatgggct
      481 gggaggcctc ctccgagcgg atgtaccccg aggacggtgc cctgaagggc aagatcaaga
      541 tgaggctgaa gctgaaggac ggcggccact acacctccga ggtcaagacc acctacaagg
      601 ccaagaagcc cgtgcagctg cccggcgcct acatcgtcga catcaagttg gacatcacct
      661 cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc cgccactcca
      721 ccggcggcat ggacgagctg tacaagtccg gatcagatct cgagctcaag cttgatatcg
      781 aattccccat ggagcagaag ctgatctccg aggaggacct gaaccggaag aaactggtgg
      841 ttgttggtga tggagcctgt ggaaagacat gcttgctcat agtcttcagc aaggacgagt
      901 tcccagaggt gtatgtgccc acagtgtttg agaactatgt ggcagatatc gaggtggatg
      961 gaaagcaggt agagttggct ttgtgggaca cagctggcca ggaggactac gaccgcctgc
     1021 ggccgctctc ctacccggac accgacgtca ttctcatgtg cttctcggtg gacagcccgg
     1081 actcgctgga gaacatcccc gagaagtggg tccccgaggt gaagcacttc tgtcccaatg
     1141 tgcccatcat cctggtggcc aacaaaaaag acctgcgcag cgacgagcat gtccgcacag
     1201 agctggcccg catgaagcag gaacccgttc gcacggatga cggccgcgcc atggccgtgc
     1261 gcatccaagc ctacgactac ctcgagtgct ctgccaagac caaggaaggc gtgcgcgagg
     1321 tcttcgagac ggccacgcgc gccgcgctgc agaagcgcta cggctcccag aacggctgca
     1381 tcaactgctg caaggtgcta tgagggccgc gcccgtcgcg cctgcccctg ccggggatcc
     1441 accggatcta gataactgat cataatcagc cataccacat tgag
//
