LOCUS       sequence-89105-e        1775 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Nucleoli, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mOrange2-Fibrillarin-7
FEATURES             Location/Qualifiers
     source          1..1775
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      28..37
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             34..741
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             complement(1537..1548)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
ORIGIN
        1 acgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggagaataac
       61 atggccatca tcaaggagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc
      121 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggctt tcagaccgct
      181 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcat
      241 ttcacctacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttcaag
      301 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga actacgagga cggcggcgtg
      361 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      421 cgcggcacca acttcccctc cgacggcccc gtgatgcaga agaagaccat gggctgggag
      481 gcctcctccg agcggatgta ccccgaggac ggtgccctga agggcaagat caagatgagg
      541 ctgaagctga aggacggcgg ccactacacc tccgaggtca agaccaccta caaggccaag
      601 aagcccgtgc agctgcccgg cgcctacatc gtcgacatca agttggacat cacctcccac
      661 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      721 ggcatggacg agctgtacaa gtccggactc agatctcgag ccatgaagcc aggattcagt
      781 ccccgtgggg gtggctttgg cggccgaggg ggctttggtg accgtggtgg tcgtggaggc
      841 cgagggggct ttggcggggg ccgaggccaa ggcggaggct ttagaggtcg tggacgagga
      901 ggaggtggag gcggcggcgg cggtggagga ggaggaagag gtggtggagg cttccatttt
      961 ggtggcaacc ggggtcgtgg tcggggagga aaaagaggaa accagtcggg gaagaatgtg
     1021 atggtggagc cgcatcggca tgagggtgtc ttcatttgtc ggggaaagga agatgcactg
     1081 gtcaccaaga acctggtccc tggggaatca gtttatggag agaagagagt ctcgatttcg
     1141 gaaggagatg acaaaattga gtaccgagcc tggaacccct tccgctccaa gctagcagca
     1201 gcaatcctgg gtggtgtgga ccagatccac atcaaaccgg gggctaaggt tctctacctc
     1261 ggggctgcct cgggcaccac ggtctcccat gtctctgaca tcgttggtcc ggatggtcta
     1321 gtctatgcag tcgagttctc ccaccgctct ggccgtgacc tcattaactt ggccaagaag
     1381 aggaccaaca tcattcctgt gatcgaggat gctcgacacc cacacaaata ccgcatgctc
     1441 atcgcaatgg tggatgtgat ctttgctgat gtggcccagc cagaccagac ccggattgtg
     1501 gccctgaatg cccacacctt cctgcgtaat ggaggacact ttgtgatttc cattaaggcc
     1561 aactgcattg actccacagc ctcagccgag gccgtgtttg cctccgaagt gaaaaagatg
     1621 caacaggaga acatgaagcc gcaggagcag ttgacccttg agccatatga aagagaccat
     1681 gccgtggtcg tgggagtgta caggccaccc cccaaggtga agaactgagg atccaccgga
     1741 tctagataac tgatcataat cagccatacc acatt
//
