LOCUS       sequence-89106-e        2177 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Centrosomes, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2177)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 2177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mOrange2-Gamma-Tubulin-17
FEATURES             Location/Qualifiers
     source          1..2177
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1423..1432
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1429..2139
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc caccatgccg agggaaatca tcaccctaca gttgggccag
       61 tgcggcaatc agattgggtt cgagttctgg aaacagctgt gcgccgagca tggtatcagc
      121 cccgagggca tcgtggagga gttcgccacc gagggcactg accacaagga cgtctttttc
      181 taccaggcag acgatgagca ctacatcccc cgggccgtgc tgctggactt ggaaccccgg
      241 gtgatccact ccatcctcaa ctccccctat gccaagctct acaacccaga gaacatctac
      301 ctgtcggaac atggaggagg agctggcaac aactgggcca gcggattctc ccagggagaa
      361 aagatccatg aggacatttt tgacatcata gaccgggagg cagatggtag tgacagtcta
      421 gagggctttg tgctgtgtca ctccattgct ggggggacag gctctggact gggttcctac
      481 ctcttagaac ggctgaatga caggtatcct aagaagctgg tgcagacata ctcagtgttt
      541 cccaaccagg acgagatgag cgatgtggtg gtccagcctt acaattcact cctcacactc
      601 aagaggctga cgcagaatgc agactgtgtg gtggtgctgg acaacacagc cctgaaccgg
      661 attgccacag accgcctgca catccagaac ccatccttct cccagatcaa ccagctggtg
      721 tctaccatca tgtcagccag caccaccacc ctgcgctacc ctggctacat gaacaatgac
      781 ctcatcggcc tcatcgcctc gctcattccc accccacggc tccacttcct catgaccggc
      841 tacacccctc tcactacgga ccagtcagtg gccagcgtga ggaagaccac ggtcctggat
      901 gtcatgaggc ggctgctgca gcccaagaac gtgatggtgt ccacaggccg agaccgccag
      961 accaaccact gctacatcgc catcctcaac atcatccagg gagaggtgga ccccacccag
     1021 gtccacaaga gcttgcagag gatccgggaa cgcaagttgg ccaacttcat cccgtggggc
     1081 cccgccagca tccaggtggc cctgtcgagg aagtctccct acctgccctc ggcccaccgg
     1141 gtcagcgggc tcatgatggc caaccacacc agcatctcct cgctcttcga gagaacctgt
     1201 cgccagtatg acaagctgcg taagcgggag gccttcctgg agcagttccg caaggaggac
     1261 atgttcaagg acaactttga tgagatggac acatccaggg agattgtgca gcagctcatc
     1321 gatgagtacc atgcggccac acggccagac tacatctcct ggggcactca ggagcagcga
     1381 attctgcagt cgacggtacc gcgggcccgg gatccaccgg tcgccaccat ggtgagcaag
     1441 ggcgaggaga ataacatggc catcatcaag gagttcatgc gcttcaaggt gcgcatggag
     1501 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag
     1561 ggctttcaga ccgctaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac
     1621 atcctgtccc ctcatttcac ctacggctcc aaggcctacg tgaagcaccc cgccgacatc
     1681 cccgactact tcaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaactac
     1741 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc
     1801 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtgat gcagaagaag
     1861 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggtgc cctgaagggc
     1921 aagatcaaga tgaggctgaa gctgaaggac ggcggccact acacctccga ggtcaagacc
     1981 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acatcgtcga catcaagttg
     2041 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc
     2101 cgccactcca ccggcggcat ggacgagctg tacaagtaag cggccgcgac tctagatcat
     2161 aatcagccat accacat
//
