LOCUS       sequence-89107-e        1027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gal-T - Golgi Complex, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 1027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mOrange2-Golgi-7
FEATURES             Location/Qualifiers
     source          1..1027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      295..304
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             301..1011
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 gagctggttt agtgaaccgt cagatccgct agcatgaggc ttcgggagcc gctcctgagc
       61 ggcagcgccg cgatgccagg cgcgtcccta cagcgggcct gccgcctgct cgtggccgtc
      121 tgcgctctgc accttggcgt caccctcgtt tactacctgg ctggccgcga cctgagccgc
      181 ctgccccaac tggtcggagt ctccacaccg ctgcagggcg gctcgaacag tgccgccgcc
      241 atcgggcagt cctccgggga gctccggacc ggaggggcca aggatccacc ggtcgccacc
      301 atggtgagca agggcgagga gaataacatg gccatcatca aggagttcat gcgcttcaag
      361 gtgcgcatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
      421 cgcccctacg agggctttca gaccgctaag ctgaaggtga ccaagggtgg ccccctgccc
      481 ttcgcctggg acatcctgtc ccctcatttc acctacggct ccaaggccta cgtgaagcac
      541 cccgccgaca tccccgacta cttcaagctg tccttccccg agggcttcaa gtgggagcgc
      601 gtgatgaact acgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
      661 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgtg
      721 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggt
      781 gccctgaagg gcaagatcaa gatgaggctg aagctgaagg acggcggcca ctacacctcc
      841 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacatcgtc
      901 gacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
      961 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagta agcggccgcg
     1021 actctag
//
