LOCUS       sequence-89108-e        1385 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1385)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mOrange2-H1-10
FEATURES             Location/Qualifiers
     source          1..1385
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      630..639
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             636..1346
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 cgtcagatcc gctagccgcc accatgaccg agaactccac ctccgccccg gcggcgaagc
       61 ccaaacgggc caaggcttcc aagaagtcca cggaccaccc caagtattca gacatgatcg
      121 tggctgctat ccaggcagag aagaaccgtg ccggctcctc gcgccagtcc atccaaaagt
      181 atatcaagag ccactacaag gtgggtgaga acgccgactc ccagatcaag ttgtccatca
      241 agcgcctagt gaccaccggt gttctcaagc aaaccaaagg ggtgggcgcc tcggggtcct
      301 tcaggctggc caagggcgat gagcccaaaa ggtcggtggc tttcaagaag accaagaagg
      361 aagtcaagaa agtggccact ccaaagaagg cagccaagcc caagaaggct gcctccaaag
      421 ccccaagcaa gaaacccaaa gccacccctg tcaagaaggc caagaagaag ccggctgcca
      481 cgcccaagaa agccaaaaag cccaaggttg tcaaagtcaa accagtcaag gcctccaaac
      541 ccaagaaggc caaaaccgtg aagcccaaag ccaagtcgag tgccaagagg gggagcaaga
      601 agaagtcggg aagcacggat ccaccggtcg ccaccatggt gagcaagggc gaggagaata
      661 acatggccat catcaaggag ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg
      721 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc tttcagaccg
      781 ctaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
      841 atttcaccta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttca
      901 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaactacgag gacggcggcg
      961 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
     1021 tgcgcggcac caacttcccc tccgacggcc ccgtgatgca gaagaagacc atgggctggg
     1081 aggcctcctc cgagcggatg taccccgagg acggtgccct gaagggcaag atcaagatga
     1141 ggctgaagct gaaggacggc ggccactaca cctccgaggt caagaccacc tacaaggcca
     1201 agaagcccgt gcagctgccc ggcgcctaca tcgtcgacat caagttggac atcacctccc
     1261 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
     1321 gcggcatgga cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccatacc
     1381 acatt
//
