LOCUS       sequence-89109-e        1195 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1195)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mOrange2-H2A-10
FEATURES             Location/Qualifiers
     source          1..1195
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      440..449
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             446..1156
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagccg ccaccatgtc gggacgtggc aagcagggcg gcaaagctcg
       61 cgccaaggcc aagacccgct cttctcgggc cgggcttcag tttcccgtag gccgagtgca
      121 tcgcctgctc cgcaaaggca actatgcgga gcgggtcggt gctggagcgc cggtgtacct
      181 ggcggcggtg ctggagtacc tgaccgccga gatcctggag ctggctggca acgcggcccg
      241 cgacaacaag aagactcgca tcatcccgcg tcacctccag ctggccatcc gcaacgatga
      301 ggagctcaac aagcttctgg gcaaagtcac catcgcacag ggtggcgtcc tgcccaacat
      361 ccaggccgtg ctactgccca agaagaccga gagccaccac aaggcgaagg gcaagtcggg
      421 aagcacggat ccaccggtcg ccaccatggt gagcaagggc gaggagaata acatggccat
      481 catcaaggag ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg gccacgagtt
      541 cgagatcgag ggcgagggcg agggccgccc ctacgagggc tttcagaccg ctaagctgaa
      601 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc atttcaccta
      661 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttca agctgtcctt
      721 ccccgagggc ttcaagtggg agcgcgtgat gaactacgag gacggcggcg tggtgaccgt
      781 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
      841 caacttcccc tccgacggcc ccgtgatgca gaagaagacc atgggctggg aggcctcctc
      901 cgagcggatg taccccgagg acggtgccct gaagggcaag atcaagatga ggctgaagct
      961 gaaggacggc ggccactaca cctccgaggt caagaccacc tacaaggcca agaagcccgt
     1021 gcagctgccc ggcgcctaca tcgtcgacat caagttggac atcacctccc acaacgagga
     1081 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     1141 cgagctgtac aagtaagcgg ccgcgactct agatcataat cagccatacc acatt
//
