LOCUS       sequence-89110-e        1223 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1223)
  AUTHORS   Kremers GJ, Hazelwood KL, Murphy CS, Davidson MW, Piston DW
  TITLE     Photoconversion in orange and red fluorescent proteins.
  JOURNAL   Nat Methods. 2009 May;6(5):355-8. doi: 10.1038/nmeth.1319. Epub 2009
            Apr 12.
  PUBMED    19363494
REFERENCE   2  (bases 1 to 1223)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1319"; journalName: "Nat Methods"; date: "2009-05"; 
            volume: "6"; issue: "5"; pages: "355-8"
COMMENT     Sequence Label: mOrange2-H2B-6
FEATURES             Location/Qualifiers
     source          1..1223
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..456
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     regulatory      469..478
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             475..1185
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattctgca
       61 gtcgacggta ccgccaccat gccagagcca gcgaagtctg ctcccgcccc gaaaaagggc
      121 tccaagaagg cggtgactaa ggcgcagaag aaaggcggca agaagcgcaa gcgcagccgc
      181 aaggagagct attccatcta tgtgtacaag gttctgaagc aggtccaccc tgacaccggc
      241 atttcgtcca aggccatggg catcatgaat tcgtttgtga acgacatttt cgagcgcatc
      301 gcaggtgagg cttcccgcct ggcgcattac aacaagcgct cgaccatcac ctccagggag
      361 atccagacgg ccgtgcgcct gctgctgcct ggggagttgg ccaagcacgc cgtgtccgag
      421 ggtactaagg ccatcaccaa gtacaccagc gctaaggatc caccggtcgc caccatggtg
      481 agcaagggcg aggagaataa catggccatc atcaaggagt tcatgcgctt caaggtgcgc
      541 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      601 tacgagggct ttcagaccgc taagctgaag gtgaccaagg gtggccccct gcccttcgcc
      661 tgggacatcc tgtcccctca tttcacctac ggctccaagg cctacgtgaa gcaccccgcc
      721 gacatccccg actacttcaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
      781 aactacgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
      841 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtgatgcag
      901 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggtgccctg
      961 aagggcaaga tcaagatgag gctgaagctg aaggacggcg gccactacac ctccgaggtc
     1021 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc
     1081 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     1141 gagggccgcc actccaccgg cggcatggac gagctgtaca agtaagcggc cgcgactcta
     1201 gatcataatc agccatacca cat
//
