LOCUS       sequence-89115-e        1336 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Light Chain Myosin, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1336)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mOrange2-LC-Myosin-N-7
FEATURES             Location/Qualifiers
     source          1..1336
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      581..590
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             587..1297
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 aacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagctta tgtcgagcaa
       61 gagagccaag gccaagacca ccaagaagag gccccagagg gctacgtcca atgtcttcgc
      121 catgtttgac cagtcccaga tccaggagtt taaggaggcc ttcaacatga ttgatcagaa
      181 ccgcgatggc ttcattgata aggaggacct gcacgacatg ttggcctctc tggggaagaa
      241 ccccacagac gagtacctgg agggcatgat gaacgaggcg ccagggccca tcaacttcac
      301 catgttcctc acaatgtttg gggagaagct gaacggcaca gaccccgagg atgtgatccg
      361 caatgccttt gcctgctttg atgaggaggc ctcaggcttc atccacgagg accacctgag
      421 ggagctgctc accaccatgg gcgaccgatt cacggatgag gaggtggacg agatgtaccg
      481 cgaggcaccc attgataaga agggcaactt caactacgtg gagttcactc gcatcctcaa
      541 acacggcgcc aaggacaagg acgacctgga tccaccggtc gccaccatgg tgagcaaggg
      601 cgaggagaat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg
      661 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
      721 ctttcagacc gctaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
      781 cctgtcccct catttcacct acggctccaa ggcctacgtg aagcaccccg ccgacatccc
      841 cgactacttc aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaactacga
      901 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
      961 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtgatgc agaagaagac
     1021 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggtgccc tgaagggcaa
     1081 gatcaagatg aggctgaagc tgaaggacgg cggccactac acctccgagg tcaagaccac
     1141 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac atcgtcgaca tcaagttgga
     1201 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     1261 ccactccacc ggcggcatgg acgagctgta caagtaagcg gccgcgactc tagatcataa
     1321 tcagccatac cacatt
//
