LOCUS       sequence-89117-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Mitochondria, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mOrange2-Mito-7
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        4..55
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     6..26
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             84..158
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      186..195
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             192..902
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 catgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gagctggttt
       61 agtgaaccgt cagatccgct agcatgtccg tcctgacgcc gctgctgctg cggggcttga
      121 caggctcggc ccggcggctc ccagtgccgc gcgccaagat ccattcgttg ggggatccac
      181 cggtcgccac catggtgagc aagggcgagg agaataacat ggccatcatc aaggagttca
      241 tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggctttc agaccgctaa gctgaaggtg accaagggtg
      361 gccccctgcc cttcgcctgg gacatcctgt cccctcattt cacctacggc tccaaggcct
      421 acgtgaagca ccccgccgac atccccgact acttcaagct gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgaac tacgaggacg gcggcgtggt gaccgtgacc caggactcct
      541 ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
      601 acggccccgt gatgcagaag aagaccatgg gctgggaggc ctcctccgag cggatgtacc
      661 ccgaggacgg tgccctgaag ggcaagatca agatgaggct gaagctgaag gacggcggcc
      721 actacacctc cgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
      781 cctacatcgt cgacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
      841 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagt
      901 aagcggccgc gactctagat cataatcagc cataccacat tgag
//
