LOCUS       sequence-89118-e        2517 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mOrange2-Paxillin-22
FEATURES             Location/Qualifiers
     source          1..2517
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    1699..1751
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      1759..1768
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1765..2475
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcac catggacgac ctcgatgcct tactggcaga cctggaatcc
       61 accacctcgc atatctctaa gcgaccggtg tttctaacag aggaaacgcc ttactcctac
      121 ccaactggaa accatacgta ccaggagatt gctgtgccac cgccggtgcc cccgccgcct
      181 tccagtgagg ccctaaatgg cactgtgatt gaccccttag accagtggca acccagcgtg
      241 tccagatacg gccaccagca acctcagtcc cagtctccga tatacagctc tagtgccaaa
      301 agctccagtg cctctgttcc tagagacggg ctcagctccc cttctccccg tgccagtgag
      361 gaggaacacg tgtacagctt cccaaacaag cagaagtctg cagaaccatc tcccacaatg
      421 accagcacct ccctgggcag caacctctcg gaactggaca ggcttctcct ggaactgaat
      481 gctgttcaac ataatccccc cagtggcttc tcagcagatg aagtcagcag aagcccatca
      541 ctgcccaatg tgaccggacc tcactatgtc atcccagaga gcagcagctc tgcaggaggg
      601 aaggctgcac cccctacaaa agaaaaacca aagcggaatg gtgggcgtgg gattgaagat
      661 gtgcgtccca gtgtggagag cctgctggat gagctggaga gctctgtgcc aagcccagtc
      721 cctgcaatca ctgtgagcca aggggaggtg agcagccccc agcgtgtcaa cgccagtcag
      781 cagcagaccc gtatctctgc ttcttcagct acacgagaac tggatgagct gatggcgtcc
      841 ctctctgact ttaagttcat ggcacagggg aaagccggcg gcagcagctc cccaccatcc
      901 accaccccaa agcctggcag tcagctggac accatgctgg gaagtctgca gtctgacctg
      961 aacaaactgg gggtagcgac ggtcgccaag ggtgtgtgcg gagcctgcaa gaagccgatt
     1021 gctgggcagg tagttacagc catggggaag acctggcacc ctgagcactt cgtctgcacc
     1081 cactgccagg aggagattgg atcacggaac ttctttgagc gggatgggca gccctactgt
     1141 gagaaggact atcacaacct cttctctcct cgctgctact actgcaatgg gccaatcctt
     1201 gataaagtgg tgacggcttt ggacaggacg tggcaccctg aacacttttt ctgtgcccag
     1261 tgtggagttt tctttggacc tgaaggattt catgagaagg atggcaaagc ctattgccgc
     1321 aaggactact ttgacatgtt tgctcccaag tgtggaggct gtgcccgggc tatcctggaa
     1381 aattacattt ctgccttaaa caccctgtgg caccctgagt gctttgtctg tcgggaatgt
     1441 ttcacaccgt tcatcaacgg cagcttcttc gagcacgacg gccagcccta ctgcgaggtg
     1501 cattaccacg agcgccgcgg ctcgctgtgc tctggctgcc agaagcccat cacgggacgc
     1561 tgcatcactg ccatgggcaa gaaatttcac cccgaacact ttgtctgtgc cttctgcctc
     1621 aagcagctca acaaaggaac cttcaaagaa cagaacgaca agccctactg tcagaactgc
     1681 tttctcaaac tcttctgtct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg
     1741 gcccgggatc caccggtcgc caccatggtg agcaagggcg aggagaataa catggccatc
     1801 atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg ccacgagttc
     1861 gagatcgagg gcgagggcga gggccgcccc tacgagggct ttcagaccgc taagctgaag
     1921 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca tttcacctac
     1981 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttcaa gctgtccttc
     2041 cccgagggct tcaagtggga gcgcgtgatg aactacgagg acggcggcgt ggtgaccgtg
     2101 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
     2161 aacttcccct ccgacggccc cgtgatgcag aagaagacca tgggctggga ggcctcctcc
     2221 gagcggatgt accccgagga cggtgccctg aagggcaaga tcaagatgag gctgaagctg
     2281 aaggacggcg gccactacac ctccgaggtc aagaccacct acaaggccaa gaagcccgtg
     2341 cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcacctccca caacgaggac
     2401 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
     2461 gagctgtaca agtaagcggc cgcgactcta gatcataatc agccatacca cattgag
//
