LOCUS       sequence-89120-e        1399 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Talin Actin Binding Domain, Excitation: 549, Emission:
            565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1399)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1399)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mOrange2-Talin-ABM-10
FEATURES             Location/Qualifiers
     source          1..1399
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             31..738
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 tcagatccgc tagcgctacc ggtcgccacc atggtgagca agggcgagga gaataacatg
       61 gccatcatca aggagttcat gcgcttcaag gtgcgcatgg agggctccgt gaacggccac
      121 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggctttca gaccgctaag
      181 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcatttc
      241 acctacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttcaagctg
      301 tccttccccg agggcttcaa gtgggagcgc gtgatgaact acgaggacgg cggcgtggtg
      361 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
      421 ggcaccaact tcccctccga cggccccgtg atgcagaaga agaccatggg ctgggaggcc
      481 tcctccgagc ggatgtaccc cgaggacggt gccctgaagg gcaagatcaa gatgaggctg
      541 aagctgaagg acggcggcca ctacacctcc gaggtcaaga ccacctacaa ggccaagaag
      601 cccgtgcagc tgcccggcgc ctacatcgtc gacatcaagt tggacatcac ctcccacaac
      661 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
      721 atggacgagc tgtacaagtc cggactcaga tctcgagctc aagcttcgat cctagaagct
      781 gccaagtcca tcgctgcagc caccagtgca ctggtaaagg ctgcgtcagc agcccagagg
      841 gagctggtgg ctcaaggaaa ggtgggcgcc attccagcca atgcactgga tgatgggcag
      901 tggtcgcagg gcctcatttc cgctgcccgt atggtggctg cggccaccaa caatctgtgt
      961 gaagcagcta acgcagctgt ccagggccat gctagccagg agaaactcat ttcctcagcc
     1021 aagcaagtag ctgcctccac agctcagctc ctggtagctt gcaaggtcaa agcagatcag
     1081 gactctgagg caatgaaacg gctccaggct gctggcaatg cagtgaagag ggcctcagat
     1141 aacctggtaa aggcggccca gaaagctgca gccttcgaag accaggagaa tgagacggtg
     1201 gtggtgaagg agaaaatggt tgggggcatt gcccagatta tcgcagcaca ggaagagatg
     1261 cttcggaagg aacgagagct ggaagaggct cggaaaaagc tcgcccagat ccggcagcag
     1321 cagtacaagt tcttgccttc agagcttcga gacgagcact aaggatccac cggatctaga
     1381 taactgatca taatcagcc
//
