LOCUS       sequence-89125-e        2230 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Intermediate Filaments, Excitation: 549, Emission: 
            565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mOrange2-Vimentin-7
FEATURES             Location/Qualifiers
     source          1..2230
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1472..1481
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1478..2188
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcccat
       61 gtccaccagg tccgtgtcct cgtcctccta ccgcaggatg ttcggcggcc cgggcaccgc
      121 gagccggccg agctccagcc ggagctacgt gactacgtcc acccgcacct acagcctggg
      181 cagcgcgctg cgccccagca ccagccgcag cctctacgcc tcgtccccgg gcggcgtgta
      241 tgccacgcgc tcctctgccg tgcgcctgcg gagcagcgtg cccggggtgc ggctcctgca
      301 ggactcggtg gacttctcgc tggccgacgc catcaacacc gagttcaaga acacccgcac
      361 caacgagaag gtggagctgc aggagctgaa tgaccgcttc gccaactaca tcgacaaggt
      421 gcgcttcctg gagcagcaga ataagatcct gctggccgag ctcgagcagc tcaagggcca
      481 aggcaagtcg cgcctggggg acctctacga ggaggagatg cgggagctgc gccggcaggt
      541 ggaccagcta accaacgaca aagcccgcgt cgaggtggag cgcgacaacc tggccgagga
      601 catcatgcgc ctccgggaga aattgcagga ggagatgctt cagagagagg aagccgaaaa
      661 caccctgcaa tctttcagac aggatgttga caatgcgtct ctggcacgtc ttgaccttga
      721 acgcaaagtg gaatctttgc aagaagagat tgcctttttg aagaaactcc acgaagagga
      781 aatccaggag ctgcaggctc agattcagga acagcatgtc caaatcgatg tggatgtttc
      841 caagcctgac ctcacggctg ccctgcgtga cgtacgtcag caatatgaaa gtgtggctgc
      901 caagaacctg caggaggcag aagaatggta caaatccaag tttgctgacc tctctgaggc
      961 tgccaaccgg aacaatgacg ccctgcgcca ggcaaagcag gagtccactg agtaccggag
     1021 acaggtgcag tccctcacct gtgaagtgga tgcccttaaa ggaaccaatg agtccctgga
     1081 acgccagatg cgtgaaatgg aagagaactt tgccgttgaa gctgctaact accaagacac
     1141 tattggccgc ctgcaggatg agattcagaa tatgaaggag gaaatggctc gtcaccttcg
     1201 tgaataccaa gacctgctca atgttaagat ggcccttgac attgagattg ccacctacag
     1261 gaagctgctg gaaggcgagg agagcaggat ttctctgcct cttccaaact tttcctccct
     1321 gaacctgagg gaaactaatc tggattcact ccctctggtt gatacccact caaaaaggac
     1381 acttctgatt aagacggttg aaactagaga tggacaggtt atcaacgaaa cttctcagca
     1441 tcacgatgac cttgaagggg atccaccggt cgccaccatg gtgagcaagg gcgaggagaa
     1501 taacatggcc atcatcaagg agttcatgcg cttcaaggtg cgcatggagg gctccgtgaa
     1561 cggccacgag ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gctttcagac
     1621 cgctaagctg aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc
     1681 tcatttcacc tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt
     1741 caagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg atgaactacg aggacggcgg
     1801 cgtggtgacc gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa
     1861 gctgcgcggc accaacttcc cctccgacgg ccccgtgatg cagaagaaga ccatgggctg
     1921 ggaggcctcc tccgagcgga tgtaccccga ggacggtgcc ctgaagggca agatcaagat
     1981 gaggctgaag ctgaaggacg gcggccacta cacctccgag gtcaagacca cctacaaggc
     2041 caagaagccc gtgcagctgc ccggcgccta catcgtcgac atcaagttgg acatcacctc
     2101 ccacaacgag gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac
     2161 cggcggcatg gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata
     2221 ccacattgag
//
