LOCUS       sequence-89126-e        4054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Focal Adhesions, Excitation: 549, Emission: 565.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4054)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 4054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mOrange2-Vinculin-23
FEATURES             Location/Qualifiers
     source          1..4054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      29..38
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             35..742
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggtcgc caccatggtg agcaagggcg aggagaataa
       61 catggccatc atcaaggagt tcatgcgctt caaggtgcgc atggagggct ccgtgaacgg
      121 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggct ttcagaccgc
      181 taagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
      241 tttcacctac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttcaa
      301 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aactacgagg acggcggcgt
      361 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
      421 gcgcggcacc aacttcccct ccgacggccc cgtgatgcag aagaagacca tgggctggga
      481 ggcctcctcc gagcggatgt accccgagga cggtgccctg aagggcaaga tcaagatgag
      541 gctgaagctg aaggacggcg gccactacac ctccgaggtc aagaccacct acaaggccaa
      601 gaagcccgtg cagctgcccg gcgcctacat cgtcgacatc aagttggaca tcacctccca
      661 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
      721 cggcatggac gagctgtaca agtccggact cagatctcga gctcaagctt cgaattcggg
      781 aaactcgaga acaaagcttg aaaacggccg catgccagtg tttcatacgc gcacgatcga
      841 gagcatcctg gagccggtgg cacagcagat ctcccacctg gtgataatgc acgaggaggg
      901 cgaggtggac ggcaaagcca ttcctgacct caccgcgccc gtggccgccg tgcaggcggc
      961 cgtcagcaac ctcgtccggg ttggaaaaga gactgttcaa accactgagg atcagatttt
     1021 gaagagagat atgccaccag catttattaa ggttgagaat gcttgcacca agcttgtcca
     1081 ggcagctcag atgcttcagt cagaccctta ctcagtgcct gctcgagatt atctaattga
     1141 tgggtcaagg ggcatcctct ctggaacatc agacctgctc cttaccttcg atgaggctga
     1201 ggtccgtaaa attattagag tttgcaaagg aattttggaa tatcttacag tggcagaggt
     1261 ggtggagact atggaagatt tggtcactta cacaaagaat cttgggccag gaatgactaa
     1321 gatggccaag atgattgacg agagacagca ggagctcact caccaggagc accgagtgat
     1381 gttggtgaac tcgatgaaca ccgtgaaaga gttgctgcca gttctcattt cagctatgaa
     1441 gatttttgta acaactaaaa actcaaaaaa ccaaggcata gaggaagctt taaaaaatcg
     1501 caattttact gtagaaaaaa tgagtgctga aattaatgag ataattcgtg tgttacaact
     1561 cacctcttgg gatgaagatg cctgggccag caaggacact gaagccatga agagagcatt
     1621 ggcctccata gactccaaac tgaaccaggc caaaggttgg ctccgtgacc ctagtgcctc
     1681 cccaggggat gctggtgagc aggccatcag acagatctta gatgaagctg gaaaagttgg
     1741 tgaactctgt gcaggcaaag aacgcaggga gattctggga acttgcaaaa tgctagggca
     1801 gatgactgat caagtggctg acctccgtgc cagaggacaa ggatcctcac cggtggccat
     1861 gcagaaagct cagcaggtat ctcagggtct ggatgtgctc acagcaaaag tggaaaatgc
     1921 agctcgcaag ctggaagcca tgaccaactc aaagcagagc attgcaaaga agatcgatgc
     1981 tgctcagaac tggcttgcag atccaaatgg tggaccggaa ggagaagagc agattcgagg
     2041 tgctttggct gaagctcgga aaatagcaga attatgtgat gatcctaaag aaagagatga
     2101 cattctacgt tcccttgggg aaatatctgc tctgacttct aaattagcag atctacgaag
     2161 acaggggaaa ggagattctc cagaggctcg agccttggcc aaacaggtgg ccacggccct
     2221 gcagaacctg cagaccaaaa ccaaccgggc tgtggccaac agcagaccgg ccaaagcagc
     2281 tgtacacctt gagggcaaga ttgagcaagc acagcggtgg attgataatc ccacagtgga
     2341 tgaccgtgga gtcggtcagg ctgccatccg ggggcttgtg gccgaagggc atcgtctggc
     2401 taatgttatg atggggcctt atcggcaaga tcttctcgcc aagtgtgacc gagtggacca
     2461 gctgacagcc cagctggctg acctggctgc cagaggggaa ggggagagtc ctcaggcacg
     2521 agcacttgca tctcagctcc aagactcctt aaaggatcta aaagctcgga tgcaggaggc
     2581 catgactcag gaagtgtcag atgttttcag cgataccaca actcccatca agctgttggc
     2641 agtggcagcc acggcgcctc ctgatgcgcc taacagggaa gaggtatttg atgagagggc
     2701 agctaacttt gaaaaccatt caggaaagct tggtgctacg gccgagaagg cggctgcggt
     2761 tggtactgct aataaatcaa cagtggaagg cattcaggcc tcagtgaaga cggcccgaga
     2821 actcacaccc caggtggtct cggctgctcg tatcttactt aggaaccctg gaaatcaagc
     2881 tgcttatgaa cattttgaga ccatgaagaa ccagtggatc gataatgttg aaaaaatgac
     2941 agggctggtg gacgaagcca ttgataccaa atctctgttg gatgcttcag aagaagcaat
     3001 taaaaaagac ctggacaagt gcaaggtagc tatggccaac attcagcctc agatgctggt
     3061 tgctggggca accagtattg ctcgtcgggc caaccggatc ctgctggtgg ctaagaggga
     3121 ggtggagaat tccgaggatc ccaagttccg tgaggctgtg aaagctgcct ctgatgaatt
     3181 gagcaaaacc atctccccga tggtgatgga tgcaaaagct gtggctggaa acatttccga
     3241 ccctggactg caaaagagct tcctggactc aggatatcgg atcctgggag ctgtggccaa
     3301 ggtcagagaa gccttccaac ctcaggagcc tgacttcccg ccgcctccac cagaccttga
     3361 acaactccga ctaacagatg agcttgctcc tcccaaacca cctctgcctg aaggtgaggt
     3421 ccctccacct aggcctccac caccagagga aaaggatgaa gagttccctg agcagaaggc
     3481 cggggaggtg attaaccagc caatgatgat ggctgccaga cagctccatg atgaagctcg
     3541 caaatggtcc agcaagggca atgacatcat tgcagcagcc aagcgcatgg ctctgctgat
     3601 ggctgagatg tctcggctgg taagaggggg cagtggtacc aagcgggcac tcattcagtg
     3661 tgccaaggac atcgccaagg cctcagatga ggtgactcgg ttggccaagg aggttgccaa
     3721 gcagtgcaca gataaacgga ttagaaccaa cctcttacag gtatgtgagc gaatcccaac
     3781 cataagcacc cagctcaaaa tcctgtccac agtgaaggcc accatgctgg gccggaccaa
     3841 catcagtgat gaggagtctg agcaggccac agagatgctg gttcacaatg cccagaacct
     3901 catgcagtct gtgaaggaga ctgtgcggga agctgaagct gcttcaatca aaattcgaac
     3961 agatgctgga tttacactgc gctgggttag aaagactccc tggtaccagt aggcggccgc
     4021 gactctagat cataatcagc cataccacat tgag
//
