LOCUS       sequence-89134-e        1065 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Endoplasmic Reticulum, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1065)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagRFP-CytERM-N-17
FEATURES             Location/Qualifiers
     source          1..1065
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      205..214
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             211..924
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 atccgctagc gctaccggga ctcagatctg gtaccccttg cagcttccag ttgtcaggaa
       61 gagaaggctc ccatggatcc tgtggtggtg ctggggctct gtctctcctg tttgcttctc
      121 ctttcactct ggaaacagag ctatggggga gggaagcttc gaattctgca gtcgacggta
      181 ccgcgggccc gggatccacc ggtcgccacc atggtgtcta agggcgaaga gctgattaag
      241 gagaacatgc acatgaagct gtacatggag ggcaccgtga acaaccacca cttcaagtgc
      301 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc
      361 gagggcggcc ctctcccctt cgccttcgac atcctggcta ccagcttcat gtacggcagc
      421 agaaccttca tcaaccacac ccagggcatc cccgacttct ttaagcagtc cttccctgag
      481 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag
      541 gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc
      601 ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggccaa caccgagatg
      661 ctgtaccccg ctgacggcgg cctggaaggc agaagcgaca tggccctgaa gctcgtgggc
      721 gggggccacc tgatctgcaa cttcaagacc acatacagat ccaagaaacc cgctaagaac
      781 ctcaagatgc ccggcgtcta ctatgtggac cacagactgg aaagaatcaa ggaggccgac
      841 aaagagacct acgtcgagca gcacgaggtg gctgtggcca gatactgcga cctccctagc
      901 aaactggggc acaaacttaa ttgagcggcc gcgactctag atcataatca gccataccac
      961 atttgtagag gttttacttg ctttaaaaac ctcccacacc tccccctgaa cctgaaacat
     1021 aaaatgatgc aatgtgtgta cttgtttatg cagctatatg ctaca
//
