LOCUS       sequence-89141-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Plasma Membrane, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagRFP-Membrane-1
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    6..62
                     /label=MCS
                     /note="multiple cloning site"
     CDS             136..822
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSDMALKL
                     VGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAVARYCD
                     LPSKLGH"
ORIGIN
        1 gactcagatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat
       61 ccaccggtcg ccaccatgct gtgctgtatg agaagaacca aacaggttga aaagaatgat
      121 gaggaccaaa agatcagcga gctgattaag gagaacatgc acatgaagct gtacatggag
      181 ggcaccgtga acaaccacca cttcaagtgc acatccgagg gcgaaggcaa gccctacgag
      241 ggcacccaga ccatgagaat caaggtggtc gagggcggcc ctctcccctt cgccttcgac
      301 atcctggcta ccagcttcat gtacggcagc agaaccttca tcaaccacac ccagggcatc
      361 cccgacttct ttaagcagtc cttccctgag ggcttcacat gggagagagt caccacatac
      421 gaagacgggg gcgtgctgac cgctacccag gacaccagcc tccaggacgg ctgcctcatc
      481 tacaacgtca agatcagagg ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa
      541 acactcggct gggaggccaa caccgagatg ctgtaccccg ctgacggcgg cctggaaggc
      601 agaagcgaca tggccctgaa gctcgtgggc gggggccacc tgatctgcaa cttcaagacc
      661 acatacagat ccaagaaacc cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac
      721 cacagactgg aaagaatcaa ggaggccgac aaagagacct acgtcgagca gcacgaggtg
      781 gctgtggcca gatactgcga cctccctagc aaactggggc acaaacttaa ttgagcggcc
      841 gcgactctag atcataatca gccataccac atgag
//
