LOCUS       sequence-89144-e        1711 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1711)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagRFP-PCNA-19-SV40NLS-4
FEATURES             Location/Qualifiers
     source          1..1711
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..49
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             53..73
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             77..97
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      104..113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             110..820
                     /codon_start=1
                     /product="monomeric derivative of red fluorescent protein 
                     from Entacmaea quadricolor (Merzlyak et al., 2007)"
                     /label=TagRFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRSD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
ORIGIN
        1 gacgtcagat ccgctagcca ccatggatcc aaaaaagaag agaaaggtag atccaaaaaa
       61 gaagagaaag gtagatccaa aaaagaagag aaaggtaccg gtcgccacca tggtgtctaa
      121 gggcgaagag ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa
      181 caaccaccac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
      241 catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac
      301 cagcttcatg tacggcagca gaaccttcat caaccacacc cagggcatcc ccgacttctt
      361 taagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg
      421 cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa
      481 gatcagaggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg
      541 ggaggccaac accgagatgc tgtaccccgc tgacggcggc ctggaaggca gaagcgacat
      601 ggccctgaag ctcgtgggcg ggggccacct gatctgcaac ttcaagacca catacagatc
      661 caagaaaccc gctaagaacc tcaagatgcc cggcgtctac tatgtggacc acagactgga
      721 aagaatcaag gaggccgaca aagagaccta cgtcgagcag cacgaggtgg ctgtggccag
      781 atactgcgac ctccctagca aactggggca caaacttaat tccggagaag ggcaagggca
      841 agggcaaggg ccgggccgcg gctacgcgta tcgatccatg ttcgaggcgc gcctggtcca
      901 gggctccatc ctcaagaagg tgttggaggc actcaaggac ctcatcaacg aggcctgctg
      961 ggatattagc tccagcggtg taaacctgca gagcatggac tcgtcccacg tctctttggt
     1021 gcagctcacc ctgcggtctg agggcttcga cacctaccgc tgcgaccgca acctggccat
     1081 gggcgtgaac ctcaccagta tgtccaaaat actaaaatgc gccggcaatg aagatatcat
     1141 tacactaagg gccgaagata acgcggatac cttggcgcta gtatttgaag caccaaacca
     1201 ggagaaagtt tcagactatg aaatgaagtt gatggattta gatgttgaac aacttggaat
     1261 tccagaacag gagtacagct gtgtagtaaa gatgccttct ggtgaatttg cacgtatatg
     1321 ccgagatctc agccatattg gagatgctgt tgtaatttcc tgtgcaaaag acggagtgaa
     1381 attttctgca agtggagaac ttggaaatgg aaacattaaa ttgtcacaga caagtaatgt
     1441 cgataaagag gaggaagctg ttaccataga gatgaatgaa ccagttcaac taacttttgc
     1501 actgaggtac ctgaacttct ttacaaaagc cactccactc tcttcaacgg tgacactcag
     1561 tatgtctgca gatgtacccc ttgttgtaga gtataaaatt gcggatatgg gacacttaaa
     1621 atactacttg gctcccaaga tcgaggatga agaaggatct tagggatcca ccggatctag
     1681 ataactgatc ataatcagcc ataccacatt t
//
