LOCUS       sequence-89151-e        1958 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Actin, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 1958)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mTagRFP-T-Actin-7
FEATURES             Location/Qualifiers
     source          1..1958
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      25..34
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             31..762
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 tcagatccgc tagcgctacc ggtcgccacc atggtgtcta agggcgaaga gctgattaag
       61 gagaacatgc acatgaagct gtacatggag ggcaccgtga acaaccacca cttcaagtgc
      121 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc
      181 gagggcggcc ctctcccctt cgccttcgac atcctggcta ccagcttcat gtacggcagc
      241 agaaccttca tcaaccacac ccagggcatc cccgatttct ttaagcagtc cttccctgag
      301 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag
      361 gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc
      421 ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggccaa caccgagatg
      481 ctgtaccccg ctgacggcgg cctggaaggc agaaccgaca tggccctgaa gctcgtgggc
      541 gggggccacc tgatctgcaa cttcaagacc acatacagat ccaagaaacc cgctaagaac
      601 ctcaagatgc ccggcgtcta ctatgtggac cacagactgg aaagaatcaa ggaggccgac
      661 aaagagacct acgtcgagca gcacgaggtg gctgtggcca gatactgcga cctccctagc
      721 aaactggggc acaaacttaa tggcatggac gagctgtaca agtccggact cagatctcga
      781 gctatggatg atgatatcgc cgcgctcgtc gtcgacaacg gctccggcat gtgcaaggcc
      841 ggcttcgcgg gcgacgatgc cccccgggcc gtcttcccct ccatcgtggg gcgccccagg
      901 caccagggcg tgatggtggg catgggtcag aaggattcct atgtgggcga cgaggcccag
      961 agcaagagag gcatcctcac cctgaagtac cccatcgagc acggcatcgt caccaactgg
     1021 gacgacatgg agaaaatctg gcaccacacc ttctacaatg agctgcgtgt ggctcccgag
     1081 gagcaccccg tgctgctgac cgaggccccc ctgaacccca aggccaaccg cgagaagatg
     1141 acccagatca tgtttgagac cttcaacacc ccagccatgt acgttgctat ccaggctgtg
     1201 ctatccctgt acgcctctgg ccgtaccact ggcatcgtga tggactccgg tgacggggtc
     1261 acccacactg tgcccatcta cgaggggtat gccctccccc atgccatcct gcgtctggac
     1321 ctggctggcc gggacctgac tgactacctc atgaagatcc tcaccgagcg cggctacagc
     1381 ttcaccacca cggccgagcg ggaaatcgtg cgtgacatta aggagaagct gtgctacgtc
     1441 gccctggact tcgagcaaga gatggccacg gctgcttcca gctcctccct ggagaagagc
     1501 tacgagctgc ctgacggcca ggtcatcacc attggcaatg agcggttccg ctgccctgag
     1561 gcactcttcc agccttcctt cctgggcatg gagtcctgtg gcatccacga aactaccttc
     1621 aactccatca tgaagtgtga cgtggacatc cgcaaagacc tgtacgccaa cacagtgctg
     1681 tctggcggca ccaccatgta ccctggcatt gccgacagga tgcagaagga gatcactgcc
     1741 ctggcaccca gcacaatgaa gatcaagatc attgctcctc ctgagcgcaa gtactccgtg
     1801 tggatcggcg gctccatcct ggcctcgctg tccaccttcc agcagatgtg gatcagcaag
     1861 caggagtatg acgagtccgg cccctccatc gtccaccgca aatgcttcta gggatccacc
     1921 ggatctagat aactgatcat aatcagccat accacatt
//
