LOCUS       sequence-89152-e        1809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1809)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagRFP-T-Annexin-12
FEATURES             Location/Qualifiers
     source          1..1809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      23..32
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..760
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 agatccgcta gcgctaccgg tcgccaccat ggtgtctaag ggcgaagagc tgattaagga
       61 gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac
      121 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggtggtcga
      181 gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcag
      241 aaccttcatc aaccacaccc agggcatccc cgatttcttt aagcagtcct tccctgaggg
      301 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
      361 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttccc
      421 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggccaaca ccgagatgct
      481 gtaccccgct gacggcggcc tggaaggcag aaccgacatg gccctgaagc tcgtgggcgg
      541 gggccacctg atctgcaact tcaagaccac atacagatcc aagaaacccg ctaagaacct
      601 caagatgccc ggcgtctact atgtggacca cagactggaa agaatcaagg aggccgacaa
      661 agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa
      721 actggggcac aaacttaatg gcatggacga gctgtacaag tccggactca gatctcgagc
      781 tcaagcttcg aattctatgg ccatggcaac caaaggaggt actgtcaaag ctgcttcagg
      841 attcaatgcc atggaagatg cccagaccct gaggaaggcc atgaaagggc tcggcaccga
      901 tgaagacgcc attattagcg tccttgccta ccgcaacacc gcccagcgcc aggagatcag
      961 gacagcctac aagagcacca tcggcaggga cttgatagac gacctgaagt cagaactgag
     1021 tggcaacttc gagcaggtga ttgtggggat gatgacgccc acggtgctgt atgacgtgca
     1081 agagctgcga agggccatga agggagccgg cactgatgag ggctgcctaa ttgagatcct
     1141 ggcctcccgg acccctgagg agatccggcg cataagccaa acctaccagc agcaatatgg
     1201 acggagcctt gaagatgaca ttcgctctga cacatcgttc atgttccagc gagtgctggt
     1261 gtctctgtca gctggtggga gggatgaagg aaattatctg gacgatgctc tcgtgagaca
     1321 ggatgcccag gacctgtatg aggctggaga gaagaaatgg gggacagatg aggtgaaatt
     1381 tctaactgtt ctctgttccc ggaaccgaaa tcacctgttg catgtgtttg atgaatacaa
     1441 aaggatatca cagaaggata ttgaacagag tattaaatct gaaacatctg gtagctttga
     1501 agatgctctg ctggctatag taaagtgcat gaggaacaaa tctgcatatt ttgctgaaaa
     1561 gctctataaa tcgatgaagg gcttgggcac cgatgataac accctcatca gagtgatggt
     1621 ttctcgagca gaaattgaca tgttggatat ccgggcacac ttcaagagac tctatggaaa
     1681 gtctctgtac tcgttcatca agggtgacac atctggagac tacaggaaag tactgcttgt
     1741 tctctgtgga ggagatgatt aaggatccac cggatctaga taactgatca taatcagcca
     1801 taccacatt
//
