LOCUS       sequence-89154-e        1344 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus/Centromeres, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1344)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1344)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagRFP-T-CENPB-N-22
FEATURES             Location/Qualifiers
     source          1..1344
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      17..26
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    530..582
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      590..599
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             596..1327
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcca ccatgggccc caagcggcgg cagctgacgt tccgggagaa
       61 gtcgcggatc atccaggagg tggaggagaa cccggaccta cgcaagggcg agatcgcgcg
      121 gcgcttcaac atcccgccgt ccacgctgag cacgatcctg aagaacaagc gcgccatcct
      181 ggcgtcggag cgcaagtacg gggtggcctc cacctgccgc aagaccaaca agctgtctcc
      241 ctacgacaag ctcgagggct tgctcatcgc ctggttccag cagatccgcg ccgccggcct
      301 gccggtcaag ggcatcatcc tcaaggagaa ggcgctgcgc atagccgagg agctgggcat
      361 ggacgacttc accgcctcca acggctggct ggaccgcttc cgccggcgcc acggcgtggt
      421 gtcctgcagc ggcgtggccc gcgcccgcgc gcgaaacgct gccccccgca ccccggcggc
      481 gcctgccagt ccggccgcgg tgccctcgga gggcagtggc gggagcactc tcgagctcaa
      541 gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccaccggtcg ccaccatggt
      601 gtctaagggc gaagagctga ttaaggagaa catgcacatg aagctgtaca tggagggcac
      661 cgtgaacaac caccacttca agtgcacatc cgagggcgaa ggcaagccct acgagggcac
      721 ccagaccatg agaatcaagg tggtcgaggg cggccctctc cccttcgcct tcgacatcct
      781 ggctaccagc ttcatgtacg gcagcagaac cttcatcaac cacacccagg gcatccccga
      841 tttctttaag cagtccttcc ctgagggctt cacatgggag agagtcacca catacgaaga
      901 cgggggcgtg ctgaccgcta cccaggacac cagcctccag gacggctgcc tcatctacaa
      961 cgtcaagatc agaggggtga acttcccatc caacggccct gtgatgcaga agaaaacact
     1021 cggctgggag gccaacaccg agatgctgta ccccgctgac ggcggcctgg aaggcagaac
     1081 cgacatggcc ctgaagctcg tgggcggggg ccacctgatc tgcaacttca agaccacata
     1141 cagatccaag aaacccgcta agaacctcaa gatgcccggc gtctactatg tggaccacag
     1201 actggaaaga atcaaggagg ccgacaaaga gacctacgtc gagcagcacg aggtggctgt
     1261 ggccagatac tgcgacctcc ctagcaaact ggggcacaaa cttaatggca tggacgagct
     1321 gtacaagtaa gcggccgcga ctct
//
