LOCUS       sequence-89157-e        1580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Shaner NC, Lin MZ, McKeown MR, Steinbach PA, Hazelwood KL, Davidson 
            MW, Tsien RY
  TITLE     Improving the photostability of bright monomeric orange and red 
            fluorescent proteins.
  JOURNAL   Nat Methods. 2008 Jun;5(6):545-51. doi: 10.1038/nmeth.1209. Epub 
            2008 May 4.
  PUBMED    18454154
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.1209"; journalName: "Nat Methods"; date: "2008-06"; 
            volume: "5"; issue: "6"; pages: "545-51"
COMMENT     Sequence Label: mTagRFP-T-Cx26-7
FEATURES             Location/Qualifiers
     source          1..1580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      798..807
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             804..1535
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 gacgtcagat ccgctagcgc taccggactc agatctcgag ctcaagcttc gaattcgcgg
       61 ctgcacgctc ctcgggacac agtgccaacc atccagagga caagatggat tggggcacac
      121 tacagagcat cctcgggggt gtcaacaagc actccaccag cattgggaaa atctggctca
      181 ctgtcctctt catcttccgc atcatgatcc tcgtggtggc cgcgaaggag gtgtggggag
      241 atgagcaagc cgattttgtt tgcaacactc tccagcctgg ctgtaagaat gtgtgctacg
      301 accactactt ccccatctct cacatccggc tctgggctct gcagctgatc atggtgtcca
      361 cgccggccct cctggtagct atgcacgtgg cctaccggag acacgaaaag aaacggaagt
      421 tcatgaaggg agagataaag aacgagttta aggacatcga agagatcaaa acccagaagg
      481 tccgtatcga agggtccctg tggtggacct acaccaccag catcttcttc cgggtcatct
      541 tcgaagctgt cttcatgtat gtcttttaca tcatgtacaa tggcttcttc atgcagcgtc
      601 tggtgaagtg taacgcctgg ccttgtccca atacagtgga ctgcttcatt tccaggccca
      661 cagaaaagac tgtcttcacg gtgttcatga tctctgtgtc tggaatttgc atcctgctaa
      721 acatcacaga gctgtgctat ctgttcatta ggtattgctc agggaagtcc aaaagaccag
      781 tcgcggatcc accggtcgcc accatggtgt ctaagggcga agagctgatt aaggagaaca
      841 tgcacatgaa gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg
      901 agggcgaagg caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg
      961 gccctctccc cttcgccttc gacatcctgg ctaccagctt catgtacggc agcagaacct
     1021 tcatcaacca cacccagggc atccccgatt tctttaagca gtccttccct gagggcttca
     1081 catgggagag agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca
     1141 gcctccagga cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcccatcca
     1201 acggccctgt gatgcagaag aaaacactcg gctgggaggc caacaccgag atgctgtacc
     1261 ccgctgacgg cggcctggaa ggcagaaccg acatggccct gaagctcgtg ggcgggggcc
     1321 acctgatctg caacttcaag accacataca gatccaagaa acccgctaag aacctcaaga
     1381 tgcccggcgt ctactatgtg gaccacagac tggaaagaat caaggaggcc gacaaagaga
     1441 cctacgtcga gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg
     1501 ggcacaaact taatggcatg gacgagctgt acaagtaagc ggccgcgact ctagatcata
     1561 atcagccata ccacattgag
//
