LOCUS       sequence-89158-e        1761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Gap Junctions, Excitation: 555, Emission: 584.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1761)
  TITLE     Davidson Orange Fluorescent Proteins
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mTagRFP-T-Cx32-7
FEATURES             Location/Qualifiers
     source          1..1761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      982..991
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             988..1719
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
ORIGIN
        1 acgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattccctct
       61 gggaaagggc agcagcagcc aggtgtggca gtgacaggga ggtgtgaatg aggcaggatg
      121 aactggacag gtttgtacac cttgctcagt ggcgtgaacc ggcattctac tgccattggc
      181 cgagtatggc tctcggtcat cttcatcttc agaatcatgg tgctggtggt ggctgcagag
      241 agtgtgtggg gtgatgagaa atcttccttc atctgcaaca cactccagcc tggctgcaac
      301 agcgtttgct atgaccaatt cttccccatc tcccatgtgc ggctgtggtc cctgcagctc
      361 atcctagttt ccaccccagc tctcctcgtg gccatgcacg tggctcacca gcaacacata
      421 gagaagaaaa tgctacggct tgagggccat ggggaccccc tacacctgga ggaggtgaag
      481 aggcacaagg tccacatctc agggacactg tggtggacct atgtcatcag cgtggtgttc
      541 cggctgttgt ttgaggccgt cttcatgtat gtcttttatc tgctctaccc tggctatgcc
      601 atggtgcggc tggtcaagtg cgacgtctac ccctgcccca acacagtgga ctgcttcgtg
      661 tcccgcccca ccgagaaaac cgtcttcacc gtcttcatgc tagctgcctc tggcatctgc
      721 atcatcctca atgtggccga ggtggtgtac ctcatcatcc gggcctgtgc ccgccgagcc
      781 cagcgccgct ccaatccacc ttcccgcaag ggctcgggct tcggccaccg cctctcacct
      841 gaatacaagc agaatgagat caacaagctg ctgagtgagc aggatggctc cctgaaagac
      901 atactgcgcc gcagccctgg caccggggct gggctggctg aaaagagcga ccgctgctcg
      961 gcctgcgcgg atccaccggt cgccaccatg gtgtctaagg gcgaagagct gattaaggag
     1021 aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt caagtgcaca
     1081 tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa ggtggtcgag
     1141 ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta cggcagcaga
     1201 accttcatca accacaccca gggcatcccc gatttcttta agcagtcctt ccctgagggc
     1261 ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc tacccaggac
     1321 accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt gaacttccca
     1381 tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccaacac cgagatgctg
     1441 taccccgctg acggcggcct ggaaggcaga accgacatgg ccctgaagct cgtgggcggg
     1501 ggccacctga tctgcaactt caagaccaca tacagatcca agaaacccgc taagaacctc
     1561 aagatgcccg gcgtctacta tgtggaccac agactggaaa gaatcaagga ggccgacaaa
     1621 gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct ccctagcaaa
     1681 ctggggcaca aacttaatgg catggacgag ctgtacaagt aagcggccgc gactctagat
     1741 cataatcagc cataccacat t
//
